Regulated expression of nullo is required for the formation of distinct apical and basal adherens junctions in the Drosophila blastoderm.
Regulated expression of nullo is required for the formation of distinct apical and basal adherens junctions in the Drosophila blastoderm.
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DOI：
10.1083/jcb.150.2.391
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发表时间：
2000-07-24
期刊：
影响因子：
--
通讯作者：
Wieschaus E
中科院分区：
文献类型：
--
作者：
Hunter C;Wieschaus E
在细胞化过程中，果蝇胚胎经历了一个大规模的细胞动力学事件，将数千个合胞体细胞核包装成单个细胞，导致胚胎皮质中上皮单层的从头形成。粘附连接的形成是在细胞化过程中建立的上皮极性的许多方面之一：在细胞化开始时，果蝇β-连环蛋白同源物Armadillo（Arm）在卵裂沟的前缘积累，随后积累到形成粘附小带前体的顶外侧区域。在这篇论文中，我们发现Arm的基底积累与DE-钙粘蛋白和Dα-连环蛋白共定位，并且对应于一个紧密膜结合的区域，我们称之为基底连接。虽然这两个连接在组分和功能上相似，但它们对新型细胞化蛋白Nullo的反应不同。Nullo存在于基底连接中，并且在细胞化开始时其形成是必需的。相反，Nullo在顶端连接形成之前被降解，并且Nullo的长期表达阻断了连接组分的顶端聚集，导致发育中胚胎的形态缺陷。这些观察结果揭示了顶端和基底连接形成的差异，并提供了Nullo在基底连接形成中的作用的见解。
During cellularization, the Drosophila embryo undergoes a large-scale cytokinetic event that packages thousands of syncytial nuclei into individual cells, resulting in the de novo formation of an epithelial monolayer in the cortex of the embryo. The formation of adherens junctions is one of the many aspects of epithelial polarity that is established during cellularization: at the onset of cellularization, the Drosophila β-catenin homologue Armadillo (Arm) accumulates at the leading edge of the cleavage furrow, and later to the apicolateral region where the zonula adherens precursors are formed. In this paper, we show that the basal accumulation of Arm colocalizes with DE-cadherin and Dα-catenin, and corresponds to a region of tight membrane association, which we refer to as the basal junction. Although the two junctions are similar in components and function, they differ in their response to the novel cellularization protein Nullo. Nullo is present in the basal junction and is required for its formation at the onset of cellularization. In contrast, Nullo is degraded before apical junction formation, and prolonged expression of Nullo blocks the apical clustering of junctional components, leading to morphological defects in the developing embryo. These observations reveal differences in the formation of the apical and basal junctions, and offer insight into the role of Nullo in basal junction formation.
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影响因子：
64.5
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