OsTCP19 influences developmental and abiotic stress signaling by modulating ABI4-mediated pathways.
OsTCP19 influences developmental and abiotic stress signaling by modulating ABI4-mediated pathways.
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OsTCP19 通过调节 ABI4 介导的途径影响发育和非生物应激信号传导。
DOI：
10.1038/srep09998
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发表时间：
2015-04-29
影响因子：
4.6
通讯作者：
Tyagi AK
中科院分区：
文献类型：
--
作者：
Mukhopadhyay P;Tyagi AK
I类TCP转录因子是植物特异性的发育调节因子。本研究探讨了水稻OsTCP19基因在水分胁迫和盐胁迫响应中的作用。除了非生物胁迫的普遍上调，这一成绩单是更丰富的耐受性比敏感的水稻基因型在早期的压力。还观察到该转录本中的小框内内含子的应力、组织和基因型依赖性保留。OsTCP 19在拟南芥中的过表达导致IAA3、ABI3和ABI4的表达上调和LOX 2的表达下调，并导致侧根形成减少等发育异常。此外，水分损失和活性氧的减少，以及脂滴在转基因植株中的超积累有助于提高幼苗建立和成熟植株的抗逆性。OsTCP19还显示在瞬时测定中直接调节水稻三酰甘油生物合成基因。在水稻寡核苷酸阵列数据库中发现与转基因中上调或下调的基因相似的基因与OsTCP19共表达。OsTCP19与OsABI 4和OsULT 1的相互作用进一步表明其在调节脱落酸途径和染色质结构中的功能。因此，OsTCP 19似乎是细胞信号传导中的重要节点，其交联应激和发育途径。
Class-I TCP transcription factors are plant-specific developmental regulators. In this study, the role of one such rice gene, OsTCP19, in water-deficit and salt stress response was explored. Besides a general upregulation by abiotic stresses, this transcript was more abundant in tolerant than sensitive rice genotypes during early hours of stress. Stress, tissue and genotype-dependent retention of a small in-frame intron in this transcript was also observed. Overexpression of OsTCP19 in Arabidopsis caused upregulation of IAA3, ABI3 and ABI4 and downregulation of LOX2, and led to developmental abnormalities like fewer lateral root formation. Moreover, decrease in water loss and reactive oxygen species, and hyperaccumulation of lipid droplets in the transgenics contributed to better stress tolerance both during seedling establishment and in mature plants. OsTCP19 was also shown to directly regulate a rice triacylglycerol biosynthesis gene in transient assays. Genes similar to those up- or downregulated in the transgenics were accordingly found to coexpress positively and negatively with OsTCP19 in Rice Oligonucleotide Array Database. Interactions of OsTCP19 with OsABI4 and OsULT1 further suggest its function in modulation of abscisic acid pathways and chromatin structure. Thus, OsTCP19 appears to be an important node in cell signaling which crosslinks stress and developmental pathways.
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影响因子：
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通讯作者：
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