Phosphorylation of GluN2B subunits of N-methyl-d-aspartate receptors in the frontal association cortex involved in morphine-induced conditioned place preference in mice
Phosphorylation of GluN2B subunits of N-methyl-d-aspartate receptors in the frontal association cortex involved in morphine-induced conditioned place preference in mice
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额叶联合皮层 N-甲基-d-天冬氨酸受体 GluN2B 亚基的磷酸化参与吗啡诱导的小鼠条件性位置偏好
DOI：
10.1016/j.neulet.2020.135470
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发表时间：
2020-11
影响因子：
2.5
通讯作者：
Tao Li
中科院分区：
文献类型：
--
作者：
Gang Chen;Wei Han;Axiang Li;Jing Wang;Jing Xiao;Xin Huang;Khosa Asif Nazir;Qing Shang;Hongyan Qian;Chuchu Qiao;Xinshe Liu;Tao Li
吗啡是世界上滥用最多的毒品之一，已经造成了严重的社会问题。前额叶皮质(FRA)在记忆形成和药物成瘾中起关键作用。N-甲基-d-天冬氨酸受体(NMDARs)在前额叶皮质(PFC)中含量丰富，许多证据表明含有GluN2B的NMDARs参与了吗啡诱导的条件性位置偏爱(CPP)。然而，GluN2B在吗啡诱导的CPP过程中在FRA中的作用还没有得到充分的研究。在本工作中，我们利用CPP动物模型，检测了磷酸化(p-)GluN2B(丝氨酸；Ser1303)在吗啡诱导的CPP不同时相FRA和NAC中的表达。我们发现p-GluN2B(Ser1303)在FRA的发展和恢复阶段是增加的，但在消亡阶段没有变化。使用GluN2B特异性拮抗剂ifenprodil阻断GluN2B在这两个时相的活性，可减弱吗啡诱导的CPP和恢复。此外，异丙苯地尔还阻断吗啡诱导的FRA中p-GluN2B(Ser1303)的上调。这些结果表明，FRA中含有GluN2B的NMDARs可能参与了对吗啡诱导的CPP的调节和恢复。
Morphine is one of the most abused drugs in the world, which has resulted in serious social problems. The frontal association cortex (FrA) has been shown to play a key role in memory formation and drug addiction.N-Methyl-d-aspartate receptors (NMDARs) are abundant in the prefrontal cortex (PFc) and much evidence indicates that GluN2B-containing NMDARs are involved in morphine-induced conditioned place preference (CPP). However, the function of GluN2B in the FrA during morphine-induced CPP has yet to be fully investigated. In the present work, a CPP animal model was employed to measure the expression of phosphorylated (p-) GluN2B (Serine; Ser 1303) in the FrA and NAc in different phases of morphine-induced CPP. We found that p-GluN2B (Ser 1303) was increased in the FrA during the development and reinstatement phases but unchanged in the extinction phase. The use of ifenprodil, a GluN2B-specific antagonist, to block the activity of GluN2B in the two phases attenuated morphine-induced CPP and reinstatement. Furthermore, ifenprodil also blocked morphine-induced upregulation of p-GluN2B (Ser 1303) in the FrA in both phases. These results indicate that GluN2B-containing NMDARs in the FrA may be involved in the regulation of morphine-induced CPP and reinstatement.
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影响因子：
2.5
作者：
Sugaya N;Ogai Y;Aikawa Y;Yumoto Y;Takahama M;Tanaka M;Haraguchi A;Umeno M;Ikeda K
通讯作者：
Ikeda K
影响因子：
3.1
作者：
Shu Y;Xu T
通讯作者：
Xu T
影响因子：
5.5
作者：
Xinshe Liu;Ying Hou;Tinglin Yan;Yan-Yan Guo-Yan;Wei Han;Fanglin Guan;Teng Chen;Tao Li
通讯作者：
Xinshe Liu;Ying Hou;Tinglin Yan;Yan-Yan Guo-Yan;Wei Han;Fanglin Guan;Teng Chen;Tao Li
影响因子：
25
作者：
Li, B;Chen, NS;Raymond, LA
通讯作者：
Raymond, LA
影响因子：
4.7
作者：
M. Vlaskovska;M. Schramm;I. Nylander;L. Kasakov;Z. You;M. Herrera-Marschitz;L. Terenius
通讯作者：
M. Vlaskovska;M. Schramm;I. Nylander;L. Kasakov;Z. You;M. Herrera-Marschitz;L. Terenius