Evidence that phosphorylation of human Upfl protein varies with intracellular location and is mediated by a wortmannin-sensitive and rapamycin-sensitive PI 3-kinase-related kinase signaling pathway.
Evidence that phosphorylation of human Upfl protein varies with intracellular location and is mediated by a wortmannin-sensitive and rapamycin-sensitive PI 3-kinase-related kinase signaling pathway.
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有证据表明，人 Upfl 蛋白的磷酸化随细胞内位置的不同而变化，并由渥曼青霉素敏感和雷帕霉素敏感的 PI 3 激酶相关激酶信号通路介导。
DOI：
10.1017/s1355838201000127
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发表时间：
2001
期刊：
影响因子：
4.5
通讯作者：
Lynne E. Maquat
中科院分区：
文献类型：
--
作者：
Mahadeb Pal;Yasuhito Ishigaki;Eszter Nagy;Lynne E. Maquat
人Upf 1蛋白（p）是一种第1组RNA解旋酶，最近被证明在哺乳动物细胞中无义介导的mRNA衰变（NMD）中发挥作用。在这里，我们证明，估计3 × 10（6）拷贝的hUpf 1 p每指数增长的HeLa细胞基本上是均匀分布在多聚核糖体，亚多聚核糖体，和核糖体的部分。我们还表明，hUpf 1 p结合RNA，是一个磷蛋白窝藏磷酸丝氨酸和磷酸苏氨酸。hUpf 1 p磷酸化程度最高时，多核糖体相关和最低程度时，核糖体自由。我们发现，血清诱导的磷酸化hUpf 1 p抑制渥曼青霉素的浓度，选择性抑制PI 3-激酶相关的激酶，并在较小程度上，雷帕霉素。这些和其他数据表明，磷酸化介导的渥曼青霉素敏感和雷帕霉素敏感的PI 3-激酶相关的激酶信号通路。将hUpf 1 p与Upf 1 p和SMG-2进行比较，它们分别是酿酒酵母和秀丽隐杆线虫中hUpf 1 p的直系同源物。
Human Upf1 protein (p), a group 1 RNA helicase, has recently been shown to function in nonsense-mediated mRNA decay (NMD) in mammalian cells. Here, we demonstrate that the estimated 3 x 10(6) copies of hUpf1 p per exponentially growing HeLa cell are essentially equally distributed among polysomal, subpolysomal, and ribosome-free fractions. We also demonstrate that hUpf1p binds RNA and is a phosphoprotein harboring phosphoserine and phosphothreonine. hUpf1p is phosphorylated to the highest extent when polysome-associated and to the lowest extent when ribosome free. We find that serum-induced phosphorylation of hUpf1p is inhibited by wortmannin at a concentration that selectively inhibits PI 3-kinase related kinases and, to a lesser extent, by rapamycin. These and other data suggest that phosphorylation is mediated by a wortmannin-sensitive and rapamycin-sensitive PI 3-kinase-related kinase signaling pathway. Comparisons are made of hUpf1p to Upf1p and SMG-2, which are the orthologs to hUpf1p in Saccharomyces cerevisiae and Caenorhabditis elegans, respectively.
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影响因子：
11.4
作者：
NIELSEN, PJ;TRACHSEL, H
通讯作者：
TRACHSEL, H
影响因子：
10.5
作者：
LEEDS, P;PELTZ, SW;CULBERTSON, MR
通讯作者：
CULBERTSON, MR
影响因子：
14.9
作者：
Applequist, SE;Selg, M;Jack, HM
通讯作者：
Jack, HM
DOI：
10.1073/pnas.93.20.10928
发表时间：
1996-10-01
影响因子：
11.1
作者：
Perlick, HA;Medghalchi, SM;Dietz, HC
通讯作者：
Dietz, HC
影响因子：
10.5
作者：
CUI, Y;HAGAN, KW;PELTZ, SW
通讯作者：
PELTZ, SW