Donor-dependent aging of young and old red blood cell subpopulations: Metabolic and functional heterogeneity.
Donor-dependent aging of young and old red blood cell subpopulations: Metabolic and functional heterogeneity.
复制标题
DOI：
10.1111/trf.16017
复制
发表时间：
2020-11
期刊：
影响因子：
2.9
通讯作者：
Acker JP
中科院分区：
文献类型：
--
作者：
Mykhailova O;Olafson C;Turner TR;DʼAlessandro A;Acker JP
红细胞（RBC）的特性受供体变异性的影响。本研究评估了来自男性和女性供体的红细胞浓缩物（RCCs）中不同生物学年龄的RBC亚群的质量和代谢组学变量，以评估其对储存损伤的贡献。将来自健康男性（n = 6）和女性（n = 4）供体的红细胞浓缩物Percoll分离成较低密度的（“年轻”，Y-RCCs）和密集型（“老的”，O-RCC）亚群，每周评估其溶血、平均细胞体积（MCV）、血红蛋白浓度（MCHC）、血红蛋白自发荧光（HGB）、形态指数（MI）、氧亲和力（p50）、刚性、细胞内活性氧（ROS）、钙（[Ca 2 +]）和基于质谱的代谢组学。具有盘状至盘状形态的年轻RCC表现出较高的MCV和MI，但较低的MCHC，HGB和刚度比O-RCC，具有盘状至球体形状。到第14天，与O-RCC相比，Y-RCC保持较低的溶血和刚性以及较高的p50。供体性别分析表明，与雄性RBC相比，雌性RBC的MCV、HGB、ROS和[Ca 2 +]较高，溶血较低，此外，第28天溶血变化率降低。代谢分析表明，在所有组中，Y-RCCs的高膜脂重塑和抗氧化能力标志物增加，而O-RCCs的氧化应激标志物增加，应对能力下降，具有显著的性别相关特征。来自女性和男性供体的Y-RCC和O-RCC的结构、功能和代谢差异证明了RCC异质性，其中来自女性的RBC对储存病变的贡献较小，并且比男性老化较慢。
Characteristics of red blood cells (RBCs) are influenced by donor variability. This study assessed quality and metabolomic variables of RBC subpopulations of varied biologic age in red blood cell concentrates (RCCs) from male and female donors to evaluate their contribution to the storage lesion. Red blood cell concentrates from healthy male (n = 6) and female (n = 4) donors were Percoll separated into less dense (“young”, Y-RCCs) and dense (“old”, O-RCCs) subpopulations, which were assessed weekly for 28 days for changes in hemolysis, mean cell volume (MCV), hemoglobin concentration (MCHC), hemoglobin autofluorescence (HGB), morphology index (MI), oxygen affinity (p50), rigidity, intracellular reactive oxygen species (ROS), calcium ([Ca2+]), and mass spectrometry–based metabolomics. Young RCCs having disc-to-discoid morphology showed higher MCV and MI, but lower MCHC, HGB, and rigidity than O-RCCs, having discoid-to-spheroid shape. By Day 14, Y-RCCs retained lower hemolysis and rigidity and higher p50 compared to O-RCCs. Donor sex analyses indicated that females had higher MCV, HGB, ROS, and [Ca2+] and lower hemolysis than male RBCs, in addition to having a decreased rate of change in hemolysis by Day 28. Metabolic profiling indicated a significant sex-related signature across all groups with increased markers of high membrane lipid remodeling and antioxidant capacity in Y-RCCs, whereas O-RCCs had increased markers of oxidative stress and decreased coping capability. The structural, functional, and metabolic dissimilarities of Y-RCCs and O-RCCs from female and male donors demonstrate RCC heterogeneity, where RBCs from females contribute less to the storage lesion and age slower than males.
登录
查看更多内容
影响因子：
2.9
作者：
D'Alessandro A;Nemkov T;Kelher M;West FB;Schwindt RK;Banerjee A;Moore EE;Silliman CC;Hansen KC
通讯作者：
Hansen KC
影响因子：
2.9
作者：
D'Alessandro A;Nemkov T;Hansen KC;Szczepiorkowski ZM;Dumont LJ
通讯作者：
Dumont LJ
影响因子：
10.1
作者：
de Wolski, Karen;Fu, Xiaoyoun;Zimring, James C.
通讯作者：
Zimring, James C.
影响因子：
7.5
作者：
Almizraq, Ruqayyah J.;Norris, Philip J.;Muszynski, Jennifer A.
通讯作者：
Muszynski, Jennifer A.
影响因子：
39
作者：
Chasse, Michael;Tinmouth, Alan;Fergusson, Dean A.
通讯作者：
Fergusson, Dean A.