Red blood cell storage in additive solution-7 preserves energy and redox metabolism: a metabolomics approach.
Red blood cell storage in additive solution-7 preserves energy and redox metabolism: a metabolomics approach.
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DOI：
10.1111/trf.13253
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发表时间：
2015-12
期刊：
影响因子：
2.9
通讯作者：
Dumont LJ
中科院分区：
文献类型：
--
作者：
D'Alessandro A;Nemkov T;Hansen KC;Szczepiorkowski ZM;Dumont LJ
红细胞的储存和输血具有巨大的医疗和经济影响。可以通过实施新型添加剂溶液（as）来改善常规储存实践，解决血库中常规冷液储存过程中生化和形态学病变的积累，例如最近引入的碱性溶液as -7。本研究假设AS-7可能通过日常储存过程中的代谢调节发挥其有益作用。红细胞在对照AS-3或实验AS-7中重悬，然后进行超高效液相色谱-质谱代谢组学分析。对229种代谢物进行了明确的定位和相对定量。AS-3和AS-7在储存过程中产生许多相似的代谢趋势，AS-7红细胞在储存的第一周代谢更活跃。as -7单元具有更快的戊糖磷酸途径燃料，更高的总谷胱甘肽库，以及通过糖酵解增加的通量，这表明了更高水平的途径中间体。代谢产物的差异在第7天尤其明显，但在42天内仍然保持不变。AS-7配方（无氯化物和碳酸氢盐负载）似乎在储存初期改善了储存红细胞的能量和氧化还原代谢，尽管差异减少，但在第42天仍然明显。能量代谢和游离脂肪酸应该作为保存红细胞结构和功能的潜在重要决定因素进行研究。未来的研究将旨在鉴定与体外和体内循环时间相关的代谢物。
Storage and transfusion of red blood cells (RBCs) has a huge medical and economic impact. Routine storage practices can be ameliorated through the implementation of novel additive solutions (ASs) that tackle the accumulation of biochemical and morphologic lesion during routine cold liquid storage in the blood bank, such as the recently introduced alkaline solution AS-7. Here we hypothesize that AS-7 might exert its beneficial effects through metabolic modulation during routine storage. Apheresis RBCs were resuspended either in control AS-3 or experimental AS-7, before ultrahigh-performance liquid chromatography–mass spectrometry metabolomics analysis. Unambiguous assignment and relative quantitation was achieved for 229 metabolites. AS-3 and AS-7 results in many similar metabolic trends over storage, with AS-7 RBCs being more metabolically active in the first storage week. AS-7 units had faster fueling of the pentose phosphate pathway, higher total glutathione pools, and increased flux through glycolysis as indicated by higher levels of pathway intermediates. Metabolite differences are especially observed at 7 days of storage, but were still maintained throughout 42 days. AS-7 formulation (chloride free and bicarbonate loading) appears to improve energy and redox metabolism in stored RBCs in the early storage period, and the differences, though diminished, are still appreciable by Day 42. Energy metabolism and free fatty acids should be investigated as potentially important determinants for preservation of RBC structure and function. Future studies will be aimed at identifying metabolites that correlate with in vitro and in vivo circulation times.
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影响因子：
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作者：
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