A mammary-specific promoter directs expression of growth hormone not only to the mammary gland, but also to Bergman glia cells in transgenic mice.
A mammary-specific promoter directs expression of growth hormone not only to the mammary gland, but also to Bergman glia cells in transgenic mice.
复制标题
乳腺特异性启动子不仅引导生长激素表达到乳腺，而且还引导到转基因小鼠的伯格曼神经胶质细胞。
DOI：
10.1210/mend-5-1-123
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发表时间：
1991
影响因子：
--
通讯作者：
G. Brem
中科院分区：
文献类型：
--
作者：
W. Günzburg;Brian Salmons;B. Zimmermann;M. Müller;V. Erfle;G. Brem
乳清酸性蛋白（WAP）启动子先前已被用于将异源基因的表达靶向转基因小鼠的乳腺。为了指导人GH（hGH）在小鼠乳腺中的表达，将hGH编码序列偶联至WAP启动子序列（WAP-hGH）。携带WAP-hGH构建体的雌性转基因小鼠在乳腺中显示hGH表达，证明了转基因的功能性。然而，当检查这些转基因小鼠的其他器官时，在所有雄性和雌性小鼠的脑中意外地观察到hGH的高水平表达。使用原位杂交或免疫组织化学，从转基因的hGH表达被认为是专门发生在伯格曼神经胶质细胞。相比之下，携带与金属硫蛋白启动子连接的hGH编码序列的小鼠在这些细胞中不表达hGH。内源性WAP基因和至少三种其它转基因（其中异源基因已置于WAP启动子的转录控制下）均不在脑中表达。因此，我们建议，WAP启动子和hGH结构基因的组合结果在一个新的组织特异性在伯格曼神经胶质细胞。
The whey acidic protein (WAP) promoter has been previously used to target the expression of heterologous genes to the mammary glands of transgenic mice. To direct the expression of human GH (hGH) to mouse mammary glands, hGH-coding sequences have been coupled to WAP promoter sequences (WAP-hGH). Female transgenic mice carrying the WAP-hGH constructs show expression of hGH in the mammary gland, demonstrating the functionality of the transgenes. However, when other organs from these transgenic mice were examined, high level expression of hGH was unexpectedly observed in the brains of all male and female mice. Using in situ hybridization or immunohistochemistry, hGH expression from the transgene was seen to occur specifically in Bergman glia cells. In contrast, mice carrying hGH-coding sequences linked to the metallothionein promoter do not express hGH in these cells. Neither the endogenous WAP gene nor at least three other transgenes in which heterologous genes have been placed under the transcriptional control of the WAP promoter are expressed in the brain. Thus, we propose that the combination of the WAP promoter and the hGH structural gene results in a novel tissue specificity in the Bergman glia.
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影响因子：
56.9
作者：
V. Pursel;Carl A. Pinkert;Kurt F. Miller;D. Bolt;R. Campbell;R. Palmiter;Ralph L. Brinster;Robert E. Hammer
通讯作者：
V. Pursel;Carl A. Pinkert;Kurt F. Miller;D. Bolt;R. Campbell;R. Palmiter;Ralph L. Brinster;Robert E. Hammer
转基因小鼠垂体中金属硫蛋白融合基因的促性腺激素特异性表达。
DOI：
10.1126/science.2868526
发表时间：
1986
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Low,MJ;Lechan,RM;Hammer,RE;Brinster,RL;Habener,JF;Mandel,G;Goodman,RH
通讯作者：
Goodman,RH
大鼠酪蛋白基因表达的复杂激素调节。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Hobbs,AA;Richards,DA;Kessler,DJ;Rosen,JM
通讯作者：
Rosen,JM
人类生长激素 DNA 序列和 mRNA 结构：可能的选择性剪接。
影响因子：
14.9
作者：
DeNoto,FM;Moore,DD;Goodman,HM
通讯作者：
Goodman,HM