Adaptation of Phaeobacter inhibens DSM 17395 to growth with complex nutrients
Adaptation of Phaeobacter inhibens DSM 17395 to growth with complex nutrients
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抑制褐杆菌 DSM 17395 适应复杂营养物质的生长
DOI：
10.1002/pmic.201200513
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发表时间：
2013
期刊：
影响因子：
3.4
通讯作者：
Schomburg
中科院分区：
文献类型：
--
作者：
Hensler;Koßmehl;Drüppel;Wöhlbrand;Trautwein;Hulsch;Maschmann;Schmidt;Reinhardt;Schmidt-Hohagen;Schomburg
本研究以玫瑰杆菌进化枝中的一个分支--在过程控制发酵罐中生长期间，与含葡萄糖的矿物培养基的参考条件相比，使用MB培养基时，P. lubensDSM 17395生长更快（μmax高3.6倍），并达到更高的光密度（2.2倍）。显然，在MB培养基的存在下，代谢被调整为以效率为代价最大化生长速率。对在1/2 OD max下收获的细胞进行的全面蛋白质组学分析鉴定出1783种（2D DIGE，膜和细胞外蛋白富集级分，鸟枪法）不同蛋白质（50.5%覆盖率），其中315种（基于2D DIGE）显示出差异丰度谱。此外，还鉴定了145种不同的代谢物（细胞内和细胞外合并），几乎所有代谢物（140种）都显示出丰度变化。在MB培养基中生长期间，P. lubensDSM 17395特异性地形成利用磷脂和几种氨基酸以及异源生成所需的各种蛋白质。对氨基酸利用的代谢调节也通过大量排出尿素来处理细胞中过量的氨来反映。显然，P. lubensDSM 17395调节其代谢，以同时利用来自复杂营养供应的多种底物。
Phaeobacter inhibensDSM 17395, a member of theRoseobacterclade, was studied for its adaptive strategies to complex and excess nutrient supply, here mimicked by cultivation with Marine Broth (MB). During growth in process‐controlled fermenters,P.inhibensDSM 17395 grew faster (3.6‐fold higher μmax) and reached higher optical densities (2.2‐fold) with MB medium, as compared to the reference condition of glucose‐containing mineral medium. Apparently, in the presence of MB medium, metabolism was tuned to maximize growth rate at the expense of efficiency. Comprehensive proteomic analysis of cells harvested at ½ ODmaxidentified 1783 (2D DIGE, membrane and extracellular protein‐enriched fractions, shotgun) different proteins (50.5% coverage), 315 (based on 2D DIGE) of which displayed differential abundance profiles. Moreover, 145 different metabolites (intra‐ and extracellular combined) were identified, almost all of which (140) showed abundance changes. During growth with MB medium,P.inhibensDSM 17395 specifically formed the various proteins required for utilization of phospholipids and several amino acids, as well as for gluconeogenesis. Metabolic tuning on amino acid utilization is also reflected by massive discharge of urea to dispose the cell of excess ammonia. Apparently,P.inhibensDSM 17395 modulated its metabolism to simultaneously utilize diverse substrates from the complex nutrient supply.
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