Resurrection genomics provides molecular and phenotypic evidence of rapid adaptation to salinization in a keystone aquatic species.
Resurrection genomics provides molecular and phenotypic evidence of rapid adaptation to salinization in a keystone aquatic species.
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DOI：
10.1073/pnas.2217276120
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发表时间：
2023-02-07
影响因子：
11.1
通讯作者：
Weider, Lawrence J.
中科院分区：
文献类型：
--
作者：
Wersebe, Matthew J.;Weider, Lawrence J.
快速适应人类引起的环境变化对于保护未来的生物多样性和生态系统服务至关重要。一个关键问题是关键物种种群是否能够快速适应以维持它们所支持的生态系统。我们研究了湖泊生态系统中主要水生草食动物溞对人为盐碱化的快速适应。通过复活几十年前的休眠卵，我们研究了一个严重盐化的湖泊在大约 25 年时间对比中的基因组变化。我们报告说，在整个基因组中显示自然选择特征的基因与渗透调节和离子调节有关。对耐盐性克隆进行表型分析揭示了基因变化可能是快速进化的基础的证据。我们提供了 D. pulicaria 快速适应盐度的分子基因组和表型证据。生态学家和进化生物学家越来越认识到野生种群的快速适应能力。快速适应人为环境变化对于维持未来的生物多样性和生态系统服务至关重要。淡水生态系统的人为盐碱化正在迅速成为主要威胁，这在北温带生态区已有充分记录。具体而言，由于城市化和导致径流的不渗透表面的增加以及道路除冰盐（例如氯化钠）的广泛使用，许多北温带湖泊已经经历了广泛的盐碱化。目前尚不清楚盐碱化的加剧是否会导致这些系统中的物种灭绝。我们利用“复活基因组学”方法，研究了美国明尼苏达州一个严重盐碱化的湖泊中的关键水生草食动物——黑水蚤是否进化出了更强的耐盐性。对来自湖中的 54 个水蚤克隆和从休眠卵孵化的代表 25 年时间对比的 54 个水蚤克隆进行全基因组重测序表明，基因组中许多含有渗透调节相关基因的区域正在研究群体中受到选择。对克隆的耐受性测定表明，最新的克隆比旧的克隆更能耐受盐分。这种模式与该湖盐度稳定的时间模式相一致。总之，我们的结果表明水蚤等关键物种可以快速适应不断增加的淡水盐碱化。此外，我们的结果表明，对盐度的快速适应可能使湖泊水蚤种群能够在人为盐碱化的情况下持续存在，从而在全球环境发生变化的情况下维持它们所支持的食物网和生态系统服务。
Rapid adaptation to human-induced environmental change is critical for preserving biodiversity and ecosystem services into the future. A key question is whether populations of keystone species can rapidly adapt to maintain the ecosystems they support. We investigated rapid adaptation to anthropogenic salinization in Daphnia pulicaria, a keystone aquatic herbivore in lake ecosystems. By resuscitating decades-old resting eggs, we investigate genomic changes across an approximately 25-y temporal contrast from a severely salinized lake. We report that the genes showing signatures of natural selection throughout the genome are related to osmoregulation and ion regulation. Phenotyping clones for salinity tolerance revealed evidence that genetic changes may underlie rapid evolution. We provide molecular genomic and phenotypic evidence for rapid adaptation to salinity in D. pulicaria. Ecologists and evolutionary biologists are increasingly cognizant of rapid adaptation in wild populations. Rapid adaptation to anthropogenic environmental change is critical for maintaining biodiversity and ecosystems services into the future. Anthropogenic salinization of freshwater ecosystems is quickly emerging as a primary threat, which is well documented in the northern temperate ecoregion. Specifically, many northern temperate lakes have undergone extensive salinization because of urbanization and the associated increase in impervious surfaces causing runoff, and the extensive use of road deicing salts (e.g., NaCl). It remains unclear whether increasing salinization will lead to extirpation of species from these systems. Using a “resurrection genomics” approach, we investigated whether the keystone aquatic herbivore, Daphnia pulicaria, has evolved increased salinity tolerance in a severely salinized lake located in Minnesota, USA. Whole-genome resequencing of 54 Daphnia clones from the lake and hatched from resting eggs that represent a 25-y temporal contrast demonstrates that many regions of the genome containing genes related to osmoregulation are under selection in the study population. Tolerance assays of clones revealed that the most recent clones are more tolerant to salinity than older clones; this pattern is concomitant with the temporal pattern of stabilizing salinity in this lake. Together, our results demonstrate that keystone species such as Daphnia can rapidly adapt to increasing freshwater salinization. Further, our results indicate that rapid adaptation to salinity may allow lake Daphnia populations to persist in the face of anthropogenic salinization maintaining the food webs and ecosystem services they support despite global environmental change.
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影响因子：
9.2
作者：
Chang CC;Chow CC;Tellier LC;Vattikuti S;Purcell SM;Lee JJ
通讯作者：
Lee JJ
影响因子：
5.2
作者：
Brans, Kristien I.;De Meester, Luc
通讯作者：
De Meester, Luc
影响因子：
4.1
作者：
Griffith MB
通讯作者：
Griffith MB
影响因子：
3.7
作者：
Aspenstrom, Pontus;Richnau, Ninna;Johansson, Ann-Sofi
通讯作者：
Johansson, Ann-Sofi
DOI：
10.1111/j.1749-6632.2010.05942.x
发表时间：
2011-01-01
期刊：
YEAR IN ECOLOGY AND CONSERVATION BIOLOGY
影响因子：
--
作者：
Findlay, Stuart E. G.;Kelly, Victoria R.
通讯作者：
Kelly, Victoria R.