Different Cis-Regulatory Elements Control the Tissue-Specific Contribution of Plastid ω-3 Desaturases to Wounding and Hormone Responses.
Different Cis-Regulatory Elements Control the Tissue-Specific Contribution of Plastid ω-3 Desaturases to Wounding and Hormone Responses.
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DOI：
10.3389/fpls.2021.727292
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发表时间：
2021
影响因子：
5.6
通讯作者：
Alfonso M
中科院分区：
文献类型：
--
作者：
Luján MA;Soria-García Á;Claver A;Lorente P;Rubio MC;Picorel R;Alfonso M
三烯脂肪酸是生物膜的重要组成部分，也是茉莉酸类化合物的前体，参与植物的防御反应。两种ω-3去饱和酶AtFAD 7和AtFAD 8在质体中合成三烯脂肪酸。启动子：GUS和诱变分析用于鉴定控制AtFAD 7和AtFAD 8基础表达的顺式元件及其对激素或创伤的响应。AtFAD 7启动子GUS活性在叶片中明显高于AtFAD 8，AtFAD 7在花的雄蕊、雌蕊、根的分生组织和维管组织中特异表达。AtFAD 7的这种特异性组织和器官表达受不同的顺式元件控制。因此，启动子缺失和突变分析表明，WRKY蛋白可能是AtFAD 7在叶片中的基础表达所必需的。AtFAD 7启动子中存在的两个MYB靶序列可能负责其在雌蕊的花雄蕊和柱头以及根分生组织中的表达，以及AtFAD 7伤口特异性反应。在AtFAD 8基因启动子的远端区域检测到的两个MYB靶序列似乎负控制AtFAD 8的表达，特别是在真叶和花中，表明MYB转录因子作为AtFAD 8基因基础表达的阻遏物，在转录水平上调节两种质体ω-3去饱和酶的不同相对丰度。我们的数据表明，在AtFAD 7启动子中检测到的两个阿坝抑制序列是功能性的，这表明ABA依赖性机制参与了两种ω-3质体去饱和酶的不同调节。这些结果揭示了不同的信号通路的协同调节拟南芥中的三烯脂肪酸含量的影响。
Trienoic fatty acids are essential constituents of biomembranes and precursors of jasmonates involved in plant defense responses. Two ω-3 desaturases, AtFAD7 and AtFAD8, synthetize trienoic fatty acids in the plastid. Promoter:GUS and mutagenesis analysis was used to identify cis-elements controlling AtFAD7 and AtFAD8 basal expression and their response to hormones or wounding. AtFAD7 promoter GUS activity was much higher than that of AtFAD8 in leaves, with specific AtFAD7 expression in the flower stamen and pistil and root meristem and vasculature. This specific tissue and organ expression of AtFAD7 was controlled by different cis-elements. Thus, promoter deletion and mutagenesis analysis indicated that WRKY proteins might be essential for basal expression of AtFAD7 in leaves. Two MYB target sequences present in the AtFAD7 promoter might be responsible for its expression in the flower stamen and stigma of the pistil and in the root meristem, and for the AtFAD7 wound-specific response. Two MYB target sequences detected in the distal region of the AtFAD8 gene promoter seemed to negatively control AtFAD8 expression, particularly in true leaves and flowers, suggesting that MYB transcription factors act as repressors of AtFAD8 gene basal expression, modulating the different relative abundance of both plastid ω-3 desaturases at the transcriptional level. Our data showed that the two ABA repression sequences detected in the AtFAD7 promoter were functional, suggesting an ABA-dependent mechanism involved in the different regulation of both ω-3 plastid desaturases. These results reveal the implication of different signaling pathways for the concerted regulation of trienoic fatty acid content in Arabidopsis.
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影响因子：
5.1
作者：
Berberich, T;Harada, M;Kusano, T
通讯作者：
Kusano, T
影响因子：
5.8
作者：
Claeys, Marleen;Storms, Valerie;Marchal, Kathleen
通讯作者：
Marchal, Kathleen
DOI：
10.1111/nph.14304
发表时间：
2017-01
期刊：
The New phytologist
影响因子：
--
作者：
Fernández-Marcos M;Desvoyes B;Manzano C;Liberman LM;Benfey PN;Del Pozo JC;Gutierrez C
通讯作者：
Gutierrez C
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
3.5
作者：
Dyer, JM;Mullen, RT
通讯作者：
Mullen, RT