Towards single-molecule nanomechanical mass spectrometry.
Towards single-molecule nanomechanical mass spectrometry.
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DOI：
10.1038/nnano.2009.152
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发表时间：
2009-07
影响因子：
38.3
通讯作者：
Roukes ML
中科院分区：
文献类型：
--
作者：
Naik AK;Hanay MS;Hiebert WK;Feng XL;Roukes ML
质谱（MS）提供了快速和定量鉴定蛋白质种类，相对较低的样品消耗。然而，随着生物分析的规模越来越小，最终缩小到单个细胞的体积，将需要具有少量到单分子灵敏度的质谱。纳米机电系统（NEMS）提供了无与伦比的质量灵敏度，现在足以实时检测单个分子物种。在这里，我们报告了基于NEMS的单生物分子检测的MS的首次演示。在我们的NEMS- ms系统中，纳米粒子和蛋白质物种通过电喷雾注入从环境条件下的流体相引入真空，随后通过六极离子光学传递到NEMS检测器。当分析物一个接一个地吸附到锁相的超高频率NEMS谐振器上时，实时记录与质量成正比的陡频移。这些第一批NEMS-MS光谱，仅从几百个质量吸附事件中获得适度的质量灵敏度，预示着这种方法的未来能力。我们还概述了短期内可行的实质性改进，其中一些是NEMS-MS独有的。
Mass spectrometry (MS) provides rapid and quantitative identification of protein species with relatively low sample consumption. Yet with the trend toward biological analysis at increasingly smaller scales, ultimately down to the volume of an individual cell, MS with few-to-single molecule sensitivity will be required. Nanoelectromechanical systems (NEMS) provide unparalleled mass sensitivity, which is now sufficient for the detection of individual molecular species in real time. Here we report the first demonstration of MS based on single-biological-molecule detection with NEMS. In our NEMS-MS system, nanoparticles and protein species are introduced by electrospray injection from fluid phase in ambient conditions into vacuum and subsequently delivered to the NEMS detector by hexapole ion optics. Precipitous frequency shifts, proportional to the mass, are recorded in real time as analytes adsorb, one-by-one, onto a phase-locked, ultrahigh frequency NEMS resonator. These first NEMS-MS spectra, obtained with modest mass sensitivity from only several hundred mass adsorption events, presage the future capabilities of this approach. We also outline the substantial improvements that are feasible in the near term, some of which are unique to NEMS-MS.
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影响因子：
3.2
作者：
Ekinci, KL;Yang, YT;Roukes, ML
通讯作者：
Roukes, ML
影响因子：
4
作者：
Ilic, B;Yang, Y;Craighead, HG
通讯作者：
Craighead, HG
影响因子：
3.3
作者：
Cleland, AN
通讯作者：
Cleland, AN
影响因子：
4
作者：
Burg, TP;Manalis, SR
通讯作者：
Manalis, SR
影响因子：
64.8
作者：
Schwab, K;Henriksen, EA;Roukes, ML
通讯作者：
Roukes, ML