The in vitro assay profile of macrocyclic lactone resistance in three species of sheep trichostrongyloids.
The in vitro assay profile of macrocyclic lactone resistance in three species of sheep trichostrongyloids.
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DOI：
10.1016/j.ijpddr.2013.04.002
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发表时间：
2013-12
影响因子：
4
通讯作者：
Sangster, Nicholas C.
中科院分区：
文献类型：
--
作者：
Demeler, Janina;Gill, Jennifer H.;von Samson-Himmelstjerna, Georg;Sangster, Nicholas C.
伊维菌素及其两种成分有助于发挥作用和产生耐药性。在LDA中，莫西菌素的耐药率往往低于伊维菌素。莫西菌素是最有效的抑制易感扭曲血吸虫迁移的药物。在检测MOX抗性方面，LMIA比LDA表现得更好。驱虫剂耐药性已成为畜牧业面临的一个重要问题。了解耐药机制，特别是对大环内酯类(MLS)的耐药机制，是开发更好的诊断工具的第一步。用幼虫发育试验(LDA)和幼虫迁移抑制试验(LMIA)对伊维菌素和米尔贝菌素等几种最低杀菌素的药效进行了测定。在三种绵羊毛状拟线虫的敏感和抗性分离株的自由生活阶段进行了这些实验。一般来说，耐药菌株的剂量反应曲线右移。数据显示，对伊维菌素及其两种成分存在耐药性，这表明这两种成分都参与了作用和耐药性。除了莫西菌素(MOX)的耐药率往往低于伊维菌素(IVM)外，受试物质对LDA中不同菌株的效力和抗药性模式并不一致。MOX是LMIA中最有效的抑制剂，对易感缠绕血吸虫的抑制作用最强，而对环状毛圆线虫的抑制作用较弱，特别是对环纹夜蛾的抑制作用更强。MOX在LMIA中均显示出较高的耐药率。基于这些结果，对MOX的耐药性具有独特的特征，LMIA可能在检测这些寄生虫对MOX的耐药性方面表现得更好。
Ivermectin and its two components contribute to action and resistance. Moxidectin tended to have lower resistance ratios than ivermectin in the LDA. Moxidectin was the most potent inhibitor of migration in susceptible H. contortus. LMIA performs better in detecting resistance to MOX than LDA. Anthelmintic resistance has emerged as an important problem in animal industries. Understanding resistance mechanisms, especially against macrocyclic lactones (MLs), is the first step in developing better diagnostic tools. Effects of several MLs including ivermectins and milbemycins were tested using two well established in vitro assays: the larval development assay (LDA) and the larval migration inhibition assay (LMIA). These were performed on free-living stages of susceptible and ML-resistant isolates of three trichostrongyloid nematode species of sheep. In general, dose response curves shifted to the right in the resistant isolates. Data showed that resistance was present to ivermectin and its two components suggesting that both components contribute to action and resistance. There were no consistent patterns of potency and resistance of the tested substances for the different isolates in the LDA except that moxidectin (MOX) tended to have lower resistance ratios than ivermectin (IVM). MOX was the most potent inhibitor in the LMIA in susceptible Haemonchus contortus while being less potent in Trichostrongylus colubriformis and particularly in Ostertagia circumcincta. MOX showed high resistance ratios in the LMIA in all three species. Based on these results, resistance to MOX has unique characteristics and the LMIA may perform better in detecting resistance to MOX in these parasite species.
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影响因子：
2.2
作者：
CONDER, GA;THOMPSON, DP;JOHNSON, SS
通讯作者：
JOHNSON, SS
影响因子：
4
作者：
Gill, JH;Lacey, E
通讯作者：
Lacey, E
影响因子：
4
作者：
GILL, JH;REDWIN, JM;LACEY, E
通讯作者：
LACEY, E
影响因子：
1.1
作者：
KIERAN, PJ
通讯作者：
KIERAN, PJ
影响因子：
2.6
作者：
Le Jambre, L;Geoghegan, J;Lyndal-Murphy, A
通讯作者：
Lyndal-Murphy, A