Natural and Genetically Engineered Proteins for Tissue Engineering.
Natural and Genetically Engineered Proteins for Tissue Engineering.
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DOI：
10.1016/j.progpolymsci.2011.07.003
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发表时间：
2012-01-01
影响因子：
27.1
通讯作者：
Kaplan DL
中科院分区：
文献类型：
--
作者：
Gomes S;Leonor IB;Mano JF;Reis RL;Kaplan DL
为了克服传统使用的自体移植、同种异体移植以及较小程度的合成材料的局限性，需要开发具有足够的机械和结构支持、控制细胞附着、迁移、增殖和分化以及具有生物可吸收特性的新一代支架。这一系列特性将使人体能够以与植入物降解相同的速度自我愈合。基因工程为生物材料系统提供了一条达到这种控制水平的途径。在自然界中表达生物成分并对其进行进一步修饰或生物工程的可能性，为多功能生物材料系统提供了一条途径。这包括产生新的蛋白质序列、新的自组装肽或不同生物活性结构域或蛋白质模体的融合的机会。可以产生具有可调特性的新的蛋白质序列，该序列可以用作新的生物材料。在这篇综述中，我们介绍了一些最常用的蛋白质在组织工程和生物医学中的应用，并描述了最常用的技术，通过将蛋白质与生物活性分子结合来提高生物性能，从而使基于蛋白质的生物材料具有功能。我们还重点介绍了利用基因工程进行蛋白质异源表达和合成新的基于蛋白质的生物聚合物，重点介绍了这些功能化生物聚合物与从自然中直接提取并通过物理吸附或化学修饰等技术进行修饰的生物聚合物相比的优势。
To overcome the limitations of traditionally used autografts, allografts and, to a lesser extent, synthetic materials, there is the need to develop a new generation of scaffolds with adequate mechanical and structural support, control of cell attachment, migration, proliferation and differentiation and with bio-resorbable features. This suite of properties would allow the body to heal itself at the same rate as implant degradation. Genetic engineering offers a route to this level of control of biomaterial systems. The possibility of expressing biological components in nature and to modify or bioengineer them further, offers a path towards multifunctional biomaterial systems. This includes opportunities to generate new protein sequences, new self-assembling peptides or fusions of different bioactive domains or protein motifs. New protein sequences with tunable properties can be generated that can be used as new biomaterials. In this review we address some of the most frequently used proteins for tissue engineering and biomedical applications and describe the techniques most commonly used to functionalize protein-based biomaterials by combining them with bioactive molecules to enhance biological performance. We also highlight the use of genetic engineering, for protein heterologous expression and the synthesis of new protein-based biopolymers, focusing the advantages of these functionalized biopolymers when compared with their counterparts extracted directly from nature and modified by techniques such as physical adsorption or chemical modification.
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DOI：
10.1002/jbm.b.31686
发表时间：
2010-10
影响因子：
3.4
作者：
Chao, Pen-Hsiu Grace;Yodmuang, Supansa;Wang, Xiaoqin;Sun, Lin;Kaplan, David L.;Vunjak-Novakovic, Gordana
通讯作者：
Vunjak-Novakovic, Gordana
影响因子：
1.8
作者：
Andrades, JA;Wu, LT;Becerra, J
通讯作者：
Becerra, J
影响因子：
7.5
作者：
BLOMBACK, B;HESSEL, B;HOGG, D
通讯作者：
HOGG, D
DOI：
10.1111/j.1582-4934.2006.tb00436.x
发表时间：
2006-07
影响因子：
5.3
作者：
Allmeling C;Jokuszies A;Reimers K;Kall S;Vogt PM
通讯作者：
Vogt PM
影响因子：
14
作者：
Amaral, Isabel F.;Unger, Ronald E.;Kirkpatrick, C. J.
通讯作者：
Kirkpatrick, C. J.