A fission yeast general translation factor reveals links between protein synthesis and cell cycle controls.
A fission yeast general translation factor reveals links between protein synthesis and cell cycle controls.
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裂殖酵母通用翻译因子揭示了蛋白质合成和细胞周期控制之间的联系。
作者：
Beáta Grallert;S. Kearsey;Michael Lenhard;Michael Lenhard;C. R. Carlson;P. Nurse;Erik Boye;Karim Labib
在两个独立的筛选中，我们分离出了裂殖酵母突变，其表型表明B-细胞周期蛋白功能或表达存在缺陷。这些突变定义了一个基因，我们称之为ded 1。我们发现，ded 1编码一个一般的翻译因子，在序列和功能相关的RNA解旋酶在其他物种中的翻译所需的。Ded 1失活后，B-细胞周期蛋白β 2和Cdc 13的水平显著降低，这种降低与后期促进复合物的降解无关。当ded 1突变体在半限制性条件下生长时，相对于其他蛋白质，Cdc 13的翻译（以及较小程度的Cdc 13）受损。我们发现，B-细胞周期蛋白翻译是特别抑制氮饥饿的野生型细胞，当B-细胞周期蛋白/Cdc 2失活是一个先决条件G（1）逮捕和随后的交配。我们的数据表明，翻译抑制B-细胞周期蛋白的表达代表了第三种机制，除了细胞周期蛋白降解和Rum 1抑制，这有助于Cdc 2失活细胞退出有丝分裂细胞周期，并准备减数分裂。
In two independent screens we isolated fission yeast mutations with phenotypes suggesting defects in B-cyclin function or expression. These mutations define a single gene which we call ded1. We show that ded1 encodes a general translation factor that is related in sequence and function to RNA helicases required for translation in other species. Levels of the B-cyclins Cig2 and Cdc13 are dramatically reduced upon inactivation of Ded1, and this reduction is independent of degradation by the anaphase promoting complex. When a ded1 mutant is grown under semi-restrictive conditions, the translation of Cig2 (and to a lesser extent Cdc13), is impaired relative to other proteins. We show that B-cyclin translation is specifically inhibited upon nitrogen starvation of wild-type cells, when B-cyclin/Cdc2 inactivation is a prerequisite for G(1) arrest and subsequent mating. Our data suggest that translational inhibition of B-cyclin expression represents a third mechanism, in addition to cyclin degradation and Rum1 inhibition, that contributes to Cdc2 inactivation as cells exit from the mitotic cell cycle and prepare for meiosis.
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影响因子：
10.5
作者：
Polymenis, M;Schmidt, EV
通讯作者：
Schmidt, EV
影响因子：
4
作者：
S. Sazer;Steven W. Sherwood
通讯作者：
S. Sazer;Steven W. Sherwood
开始即是结束：早期发育过程中 Poly(A) 添加和去除的调节。
影响因子：
13.8
作者：
Wickens,M
通讯作者：
Wickens,M
cdc25p 过度表达的抑制剂确定了影响裂殖酵母中 G2/M 检查点的两条途径。
影响因子：
3.3
作者：
Forbes,KC;Humphrey,T;Enoch,T
通讯作者：
Enoch,T
DOI：
10.1073/pnas.93.24.13837
发表时间：
1996-11-26
影响因子：
11.1
作者：
Gruidl, ME;Smith, PA;Bennett, KL
通讯作者：
Bennett, KL