Cyclodextrin-Promoted Fluorescence Detection of Aromatic Toxicants and Toxicant Metabolites in Commercial Milk Products.
Cyclodextrin-Promoted Fluorescence Detection of Aromatic Toxicants and Toxicant Metabolites in Commercial Milk Products.
复制标题
DOI：
10.1007/s12161-018-1228-8
复制
发表时间：
2018-09
影响因子：
2.9
通讯作者：
Levine M
中科院分区：
文献类型：
--
作者：
DiScenza DJ;Lynch J;Verderame M;Smith MA;Levine M
由于多环芳烃（PAHs）具有持久性、致癌性和毒性，食品和农业中PAHs及其代谢产物的检测是一个重要的研究目标。在泌乳奶牛的牛奶中，以及在多环芳烃污染地区种植的植物的叶子和茎中发现了多环芳烃，从而使它们进入牛奶和植物奶替代品。本文报道的是快速，灵敏，和选择性检测10多环芳烃和多环芳烃代谢物在各种牛奶和植物奶替代品使用荧光能量转移从多环芳烃的高量子产率荧光团，结合随后的阵列为基础的统计分析的荧光发射信号。该系统具有高灵敏度（低微摩尔检测限），选择性（即使在结构相似的分析物之间也能100%区分）和普遍适用性（对于未修饰的亲脂性PAH和高极性氧化PAH代谢物，以及不同的牛奶和植物牛奶样品）。这些有前途的结果显示出巨大的潜力，被转化为固态设备的快速，灵敏和选择性检测多环芳烃及其代谢产物在复杂的，商业食品。
The detection of polycyclic aromatic hydrocarbons (PAHs) and their metabolites in food and in agricultural sources is an important research objective due to the PAHs’ known persistence, carcinogenicity, and toxicity. PAHs have been found in the milk of lactating cows, and in the leaves and stems of plants grown in PAH-contaminated areas, thereby making their way into both cow milk and plant milk alternatives. Reported herein is the rapid, sensitive, and selective detection of 10 PAHs and PAH metabolites in a variety of cow milks and plant milk alternatives using fluorescence energy transfer from the PAH to a high quantum yield fluorophore, combined with subsequent array-based statistical analyses of the fluorescence emission signals. This system operates with high sensitivity (low micromolar detection limits), selectivity (100% differentiation even between structurally similar analytes), and general applicability (for both unmodified lipophilic PAHs and highly polar oxidized PAH metabolites, as well as for different cow and plant milk samples). These promising results show significant potential to be translated into solid-state devices for the rapid, sensitive, and selective detection of PAHs and their metabolites in complex, commercial food products.
登录
查看更多内容
影响因子：
15
作者：
Bajaj, Avinash;Miranda, Oscar R.;Phillips, Ronnie;Kim, Ik-Bum;Jerry, D. Joseph;Bunz, Uwe H. F.;Rotello, Vincent M.
通讯作者：
Rotello, Vincent M.
DOI：
10.1016/j.msec.2017.03.151
发表时间：
2017-07-01
影响因子：
7.9
作者：
Dai, Lin;Liu, Kefeng;Lei, Jiandu
通讯作者：
Lei, Jiandu
影响因子：
1.7
作者：
DiScenza, Dana J.;Verderame, Molly;Levine, Mindy
通讯作者：
Levine, Mindy
影响因子：
3.3
作者：
DiScenza, Dana J.;Levine, Mindy
通讯作者：
Levine, Mindy
影响因子：
3.3
作者：
Ghosh, Prasun;Mandal, Soumik;Purkayastha, Pradipta
通讯作者：
Purkayastha, Pradipta