A fusion protein of hepatocyte growth factor enhances reconstruction of myocardium in a cardiac patch derived from porcine urinary bladder matrix.
A fusion protein of hepatocyte growth factor enhances reconstruction of myocardium in a cardiac patch derived from porcine urinary bladder matrix.
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DOI：
10.1016/j.jtcvs.2008.07.008
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发表时间：
2008-11
影响因子：
6
通讯作者：
Zenati, Marco A.
中科院分区：
文献类型：
--
作者：
Ota, Takeyoshi;Gilbert, Thomas W.;Schwartzman, David;McTiernan, Charles F.;Kitajima, Takashi;Ito, Yoshihiro;Sawa, Yoshiki;Badylak, Stephen F.;Zenati, Marco A.
我们试图在猪模型中使用膀胱基质（UBM）结合肝细胞生长因子（HGF）和纤维连接蛋白胶原结合结构域（CBD）的融合蛋白来促进心肌修复。CBD作为中介促进HGF结合，增强HGF在UBM内的稳定性。将合并CBD-HGF的UBM植入猪右心室壁（f组）以修复手术造成的缺损。未处理UBM贴片（u组）和涤纶贴片（d组）作为对照组（N=5/组）。术后60天进行机电测绘。线性局部缩短（LLS）用于评估区域收缩性，并记录电活动。F组LLS较对照组明显改善（F:0.51±1.57%*,U: - 1.06±1.84%,D: - 2.72±2.59%,*p<0.05），但低于正常心肌（13.7±4.3%）*。F组平均电活动为1.49±0.82mV，显著高于对照组（U:0.93±0.71mV; D:0.30±0.22mV）*，显著低于正常心肌（8.24±2.49mV）*。组织学检查以α-平滑肌肌动蛋白阳性细胞为主，F组细胞层最厚，D组细胞层最薄，有心内膜内皮单层。α-肌动蛋白阳性细胞的分散孤立岛仅在F组中观察到，而在对照组中没有，提示心肌细胞的存在。与单独的UBM或涤纶相比，CBD-HGF-UBM贴片显示出更高的收缩性和电活动，并促进了宿主细胞的均匀再生。合并CBD-HGF的UBM可能有助于心肌重构。
We sought to promote myocardial repair using urinary bladder matrix (UBM) incorporated with a fusion protein which combined hepatocyte growth factor (HGF) and fibronectin collagen-binding-domain (CBD) in a porcine model. CBD acted as an intermediary to promote HGF binding and enhance HGF stability within UBM. UBM incorporated with CBD-HGF was implanted into the porcine right ventricular wall (F-group) to repair a surgically created defect. Untreated UBM patches (U-group) and Dacron patches (D-group) served as controls (N=5/group). Electromechanical mapping was performed at 60-days after surgery. Linear local shortening (LLS) was used to assess regional contractility, and electrical activity was recorded. The LLS was significantly improved in the F group compared with controls (F:0.51 ± 1.57%*, U:− 1.06 ± 1.84%, D: −2.72 ± 2.59%; *p<0.05), while they were inferior to the normal myocardium(13.7 ± 4.3%)*. Mean electrical activity was 1.49±0.82mV in the F group, which was statistically greater than control groups (U:0.93 ± 0.71mV; D:0.30 ± 0.22mV)* and less than the normal myocardium (8.24±2.49mV)*. Histological examination showed predominant α-smooth muscle actin positive cells with the F group showing the thickest layer and the D group the thinnest layer, with an endocardial endothelial monolayer. Scattered isolated islands of α-actinin positive cells were observed only in the F group, but not in the controls, suggesting the presence of cardiomyocytes. The CBD-HGF-UBM patch demonstrated increased contractility and electrical activity compared to UBM alone or Dacron, and facilitated a homogeneous repopulation of host cells. UBM incorporated with CBD-HGF may contribute to constructive myocardial remodeling.
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DOI：
10.1083/jcb.111.6.2427
发表时间：
1990-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Lyons GE;Schiaffino S;Sassoon D;Barton P;Buckingham M
通讯作者：
Buckingham M
DOI：
10.1097/01.rvi.0000058410.01661.62
发表时间：
2003-03-01
影响因子：
2.9
作者：
Brountzos, E;Pavcnik, D;Rösch, J
通讯作者：
Rösch, J
DOI：
10.1097/00002480-199407000-00107
发表时间：
1994-07-01
期刊：
ASAIO journal (American Society for Artificial Internal Organs : 1992)
影响因子：
--
作者：
Takimoto, Y;Teramachi, M;Shimizu, Y
通讯作者：
Shimizu, Y
影响因子：
37.8
作者：
Gepstein, L;Goldin, A;Ben-Haim, SA
通讯作者：
Ben-Haim, SA
影响因子：
20.1
作者：
Halbach, Marcel;Pfannkuche, Kurt;Mueller-Ehmsen, Jochen
通讯作者：
Mueller-Ehmsen, Jochen