The migration and fusion events related to ROCK activity strongly influence the morphology of chicken embryo intestinal organoids.
The migration and fusion events related to ROCK activity strongly influence the morphology of chicken embryo intestinal organoids.
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DOI：
10.1007/s00709-018-1312-3
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发表时间：
2019-03
期刊：
影响因子：
2.9
通讯作者：
Grabacka M
中科院分区：
文献类型：
--
作者：
Pierzchalska M;Panek M;Grabacka M
The method of organoid culture has become a tool widely used in gastrointestinal research, but so far, the migration of organoids derived from gut epithelium and formed in 3D Matrigel matrix has not been reported and studied. The intestinal epithelial tissue derived from 19-day-old chicken embryo was cultured in Matrigel and the dynamic properties of the forming organoids were analyzed by time-lapse image analysis. It was observed that about one in ten organoids actively moved through the matrix, at a speed of 10–20 μm/h. Moreover, rotation was observed in the majority of organoids that did not migrate long distances. The fusion events took place between organoids, which collided during the movement or growth. In our previous paper, we showed that the presence of Toll-like receptor 4 ligand, Escherichia coli lipopolysaccharide (LPS, 1 μg/ml), increased the mean organoid diameter. Here, we confirm this result and demonstrate that the Rho-associated protein kinase (ROCK) inhibitor Y-27632 (10 μM) did not completely abolish organoid migration, but prevented the fusion events, in both LPS-treated and untreated cultures. In consequence, in the presence of Y-27632, the differences between cultures incubated with and without LPS were not visible. We conclude that migration and fusion of organoids may influence their morphology and suggest that these phenomena should be taken into account during the design of experimental settings. The online version of this article (10.1007/s00709-018-1312-3) contains supplementary material, which is available to authorized users.
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影响因子：
5.5
作者：
Hiraiwa T;Kuranaga E;Shibata T
通讯作者：
Shibata T
影响因子：
--
作者：
Plutoni C;Bazellières E;Gauthier-Rouvière C
通讯作者：
Gauthier-Rouvière C
I型胶原蛋白作为人类小肠上皮体外生长的细胞外基质。
影响因子：
3.7
作者：
Jabaji Z;Brinkley GJ;Khalil HA;Sears CM;Lei NY;Lewis M;Stelzner M;Martín MG;Dunn JC
通讯作者：
Dunn JC
DOI：
10.1007/7651_2016_15
发表时间：
2019-01-01
期刊：
ORGANOIDS
影响因子：
--
作者：
Pierzchalska, Malgorzata;Panek, Malgorzata;Grabacka, Maja
通讯作者：
Grabacka, Maja
影响因子：
2.2
作者：
Panek M;Grabacka M;Pierzchalska M
通讯作者：
Pierzchalska M