Contextual processing elicits sex differences in dorsal hippocampus activation following footshock and context fear retrieval.
Contextual processing elicits sex differences in dorsal hippocampus activation following footshock and context fear retrieval.
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DOI：
10.1016/j.bbr.2020.112771
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发表时间：
2020-09-01
影响因子：
2.7
通讯作者：
Poulos AM
中科院分区：
文献类型：
--
作者：
Colon LM;Poulos AM
建立一个环境的上下文表示将特定的时空处理要求哺乳动物海马体，一个区域显示性别差异的处理能力。然而，性别差异的证据在这些处理的要求在上下文的恐惧学习仍然有限。在这里，我们研究了上下文处理之间的关系，时间的足电击，和激活的背海马和基底外侧杏仁核（BLA）在雄性和雌性小鼠（C57 Bl/6 J）。我们修改了初始暴露时间的调节上下文之前，管理，或没有，一个单一的脚电击。然后，我们定量了在背侧CA 1（近端-远端区域）、CA 3、齿状回和基底外侧杏仁核的头侧半部分中通过获取或检索上下文恐惧记忆而激活的Fos-ir神经元，其对应于艾伦参考图谱的图谱水平.在实验1中，我们发现，性别差异的背景下引起冻结是显而易见的，在最长的背景放置到休克的时间间隔和上下文恐惧检索增加上下文曝光时间增加CA 1 Fos-IR的男性，但不是女性。在实验2中，我们观察到，一个令人厌恶的足电击在男性增强CA 1激活，而它下调CA 1激活的女性。我们还发现，一个令人厌恶的足电击独立于性别调节齿状回激活，通常表现出增加激活更大的背景曝光时间。最后，我们确定了BLA神经元的反应性提高，无论是footshock和长度的背景下，女性相比，男性曝光。总的来说，我们的研究结果表明，背景恐惧条件反射的性别差异可能源于背侧海马子场的背景处理需求的显着性别差异，主要是由厌恶的结果调制。
Establishing a contextual representation of an environment places specific spatial-temporal processing demands on the mammalian hippocampus, a region showing sex-differences in processing capabilities. However, evidence for sex differences in these processing demands during contextual fear learning remains limited. Here, we examined the relationship among contextual processing, timing of footshock, and activation of the dorsal hippocampus and basolateral amygdalar nuclei (BLA) in male and female mice (C57Bl/6J). We modified the initial exposure time to the conditioning context prior to administration, or not, of a single footshock. We then quantified Fos- ir neurons activated by acquisition or retrieval of contextual fear memories in the rostral half of the dorsal CA1 (proximal – distal regions), CA3, Dentate Gyrus and basolateral amygdalar nuclei corresponding to atlas levels of the Allen Reference Atlas. In experiment 1, we found that sex differences in context elicited freezing were evident at the longest context placement-to-shock interval and that context fear retrieval with increasing contextual exposure periods increased CA1 Fos-ir in males, but not females. In experiment 2, we observed that an aversive footshock in males potentiated CA1 activation, while it downregulated CA1 activation in females. We also found that an aversive footshock independent of sex moderated Dentate Gyrus activation that normally showed increased activation with greater context exposure periods. Lastly, we identified a heightened responsiveness in BLA neurons to both footshock and length of context exposure in females compared to males. Overall, our findings suggest that sex differences in contextual fear conditioning may arise from marked sex differences in the contextual processing demands of the dorsal hippocampus subfields largely modulated by aversive outcomes.
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