Transcriptional regulator Sar regulates the multiple secretion systems in Xanthomonas oryzae.
Transcriptional regulator Sar regulates the multiple secretion systems in Xanthomonas oryzae.
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DOI：
10.1111/mpp.13272
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发表时间：
2023-01
影响因子：
4.9
通讯作者：
Ruan, Lifang
中科院分区：
文献类型：
--
作者：
Shao, Yanan;Tang, Guiyu;Huang, Yuanyuan;Ke, Wenli;Wang, Shasha;Zheng, Dehong;Ruan, Lifang
水稻白白叶枯病菌（Xoo）是一种引起水稻品种叶枯病的植物病原菌。病原细菌具有许多转录调控因子，可以调控植物的致病性等多种生物学过程。我们先前的研究鉴定了一个新的主调节因子PXO_RS20790，其参与Xoo对宿主水稻的致病性。然而，PXO_RS20790的分子功能尚不清楚。在这里，我们证明了转录调节因子Sar（PXO_RS20790）调节多个分泌系统。RNA测序分析、细菌单杂交分析和电泳迁移率变动分析显示，Sar能够结合T1 SS相关基因、无毒基因raxX的启动子，并正向调节这些基因的表达。同时，我们发现Sar对T6 SS-1簇有正调节作用，但对T6 SS-2簇没有调节作用。此外，我们发现只有T6 SS-2参与细菌间的竞争。我们还发现，Sar可以与T3 SS调节子hrpG和hrpX的启动子结合，从而激活这两个基因的转录。我们的研究结果表明，SARS是一个重要的调节器的多分泌系统和毒力。SAR是一种主要的转录调节因子，调节I型、III型和VI型分泌系统。
Xanthomonas oryzae pv. oryzae (Xoo) is a notorious plant pathogen that causes leaf blight of rice cultivars. The pathogenic bacteria possess numerous transcriptional regulators to regulate various biological processes, such as pathogenicity in the host plant. Our previous study identified a new master regulator PXO_RS20790 that is involved in pathogenicity for Xoo against the host rice. However, the molecular functions of PXO_RS20790 are still unclear. Here, we demonstrate that transcriptional regulator Sar (PXO_RS20790) regulates multiple secretion systems. The RNA‐sequencing analysis, bacterial one‐hybrid assay, and electrophoretic mobility shift assay revealed that Sar enables binding of the promoters of the T1SS‐related genes, the avirulence gene, raxX, and positively regulates these genes' expression. Meanwhile, we found that Sar positively regulated the T6SS‐1 clusters but did not regulate the T6SS‐2 clusters. Furthermore, we revealed that only T6SS‐2 is involved in interbacterial competition. We also indicated that Sar could bind the promoters of the T3SS regulators, hrpG and hrpX, to activate these two genes' transcription. Our findings revealed that Sar is a crucial regulator of multiple secretion systems and virulence. Sar, a master transcriptional regulator, regulates the type I, type III, and type VI secretion systems.
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