Oral Microbiota Shift after 12-Week Supplementation with Lactobacillus reuteri DSM 17938 and PTA 5289; A Randomized Control Trial.
Oral Microbiota Shift after 12-Week Supplementation with Lactobacillus reuteri DSM 17938 and PTA 5289; A Randomized Control Trial.
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DOI：
10.1371/journal.pone.0125812
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Johansson I
中科院分区：
文献类型：
--
作者：
Romani Vestman N;Chen T;Lif Holgerson P;Öhman C;Johansson I
乳杆菌属通过调节细菌生物膜的形成可能有助于健康，但它们对整体口腔微生物群的影响仍不清楚。每天食用罗伊氏乳杆菌 DSM 17938 和 PTA 5289 12 周后，通过对 16S rDNA 高变区 V3-V4 进行 454 焦磷酸测序来表征口腔微生物群。 44 名成年人被分配到接受乳酸杆菌含片（每种菌株/含片 108 CFU）的测试组（n = 22）或接受安慰剂的对照组（n = 22）。通过培养和物种特异性 PCR 证实了罗伊氏乳杆菌的存在。通过焦磷酸测序分析了暴露前、暴露期间和暴露后 16 名成年人的牙齿生物膜样本。总共 1,310,292 个序列经过质量过滤。删除单个读数后，人类口腔微生物组数据库中鉴定出 257 个物种或系统发育型，同一性达 98.5%。厚壁菌门、拟杆菌门、梭杆菌门、变形菌门和放线菌门是最丰富的门。链球菌属是最常见的属，口腔链球菌属是最常见的。米蒂斯/S。米蒂斯 bv2/S。婴儿组是优势种。观察到的物种数量不受罗伊氏乳杆菌暴露的影响。然而，食用罗伊氏乳杆菌的受试者在相对于基线散射的主坐标分析和焦磷酸测序微生物群的多变量建模中被聚类，并且培养和 PCR 检测到罗伊氏乳杆菌将基线与测试受试者的 12 周样本分开。罗伊氏乳杆菌摄入量与口链球菌/S 增加相关。米蒂斯/S。米蒂斯 bv2/S。婴儿组和弯曲弯曲杆菌、Granulicatella adiacens、Bergeyella sp。 HOT322、亚黄奈瑟菌和 SR1 [G-1] sp。 HOT874 检测并减少了变形链球菌、咽峡炎链球菌、粘膜奈瑟菌、周期性梭杆菌、具核梭杆菌和斑状普雷沃氏菌的检测。这种效应在暴露终止后 1 个月消失。 食用罗伊氏乳杆菌并不会影响物种丰富度，但会引起口腔微生物群组成的变化。其生物学相关性仍有待阐明。临床试验.gov NCT02311218
Lactobacillus spp. potentially contribute to health by modulating bacterial biofilm formation, but their effects on the overall oral microbiota remain unclear. Oral microbiota was characterized via 454-pyrosequencing of the 16S rDNA hypervariable region V3-V4 after 12 weeks of daily Lactobacillus reuteri DSM 17938 and PTA 5289 consumption. Forty-four adults were assigned to a test group (n = 22) that received lactobacilli lozenges (108 CFU of each strain/lozenge) or a control group that received placebo (n = 22). Presence of L. reuteri was confirmed by cultivation and species specific PCR. Tooth biofilm samples from 16 adults before, during, and after exposure were analyzed by pyrosequencing. A total of 1,310,292 sequences were quality filtered. After removing single reads, 257 species or phylotypes were identified at 98.5% identity in the Human Oral Microbiome Database. Firmicutes, Bacteroidetes, Fusobacteria, Proteobacteria, and Actinobacteria were the most abundant phyla. Streptococcus was the most common genus and the S. oralis/S. mitis/S. mitis bv2/S. infantis group comprised the dominant species. The number of observed species was unaffected by L. reuteri exposure. However, subjects who had consumed L. reuteri were clustered in a principal coordinates analysis relative to scattering at baseline, and multivariate modeling of pyrosequencing microbiota, and culture and PCR detected L. reuteri separated baseline from 12-week samples in test subjects. L. reuteri intake correlated with increased S. oralis/S. mitis/S. mitis bv2/S. infantis group and Campylobacter concisus, Granulicatella adiacens, Bergeyella sp. HOT322, Neisseria subflava, and SR1 [G-1] sp. HOT874 detection and reduced S. mutans, S. anginosus, N. mucosa, Fusobacterium periodicum, F. nucleatum ss vincentii, and Prevotella maculosa detection. This effect had disappeared 1 month after exposure was terminated. L. reuteri consumption did not affect species richness but induced a shift in the oral microbiota composition. The biological relevance of this remains to be elucidated. ClinicalTrials.gov NCT02311218
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影响因子：
5.1
作者：
Coccorullo, Paola;Strisciuglio, Caterina;Staiano, Annamaria
通讯作者：
Staiano, Annamaria
影响因子：
3.4
作者：
Caglar, E.;Kavaloglu, S. C.;Twetman, S.
通讯作者：
Twetman, S.
影响因子：
5.1
作者：
Indrio, Flavia;Riezzo, Giuseppe;Francavilla, Ruggiero
通讯作者：
Francavilla, Ruggiero
影响因子：
2.4
作者：
Bylesjo, Max;Rantalainen, Mattias;Trygg, Johan
通讯作者：
Trygg, Johan
影响因子：
11
作者：
Griffen, Ann L.;Beall, Clifford J.;Leys, Eugene J.
通讯作者：
Leys, Eugene J.