Androgen concentrations in umbilical cord blood and their association with maternal, fetal and obstetric factors.
Androgen concentrations in umbilical cord blood and their association with maternal, fetal and obstetric factors.
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DOI：
10.1371/journal.pone.0042827
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Hickey M
中科院分区：
文献类型：
--
作者：
Keelan JA;Mattes E;Tan H;Dinan A;Newnham JP;Whitehouse AJ;Jacoby P;Hickey M
本研究的目的是使用高度特异性的液相色谱-串联质谱 (LC-MS/MS) 测定法测量出生队列中的脐血雄激素浓度，并评估性别、分娩和孕龄对出生时胎儿雄激素水平的影响。我们对来自西澳大利亚一般产科人群的 803 名未经选择的单胎妊娠的混合动脉和静脉脐带血清中的雄激素浓度进行了前瞻性队列研究。从存档的脐带血清样本中提取总睾酮 (TT)、Δ4-雄烯二酮和脱氢表雄酮，并使用 LC-MS/MS 进行测量。通过ELISA测定SHBG；还计算了游离睾酮（FT）和生物可利用睾酮（BioT）值。所有三种雄激素的中值通常低于之前公布的值。男性新生儿的 TT、FT、BioT 和 SHBG 水平显着高于女性新生儿 (P<0.0001)，而女性新生儿的脱氢表雄酮水平较高 (P<0.0001)。分娩与中位脐带血 TT 和 FT 水平（男女合计）显着下降（约 15-26%）相关，但 SHBG、Δ4-雄烯二酮和脱氢表雄酮浓度适度增加（约 16-31%）。 TT和FT与分娩胎龄显着负相关，而SHBG、Δ4-雄烯二酮和脱氢表雄酮则呈正相关。产前糖皮质激素给药在多元回归模型中也有显着影响。这是第一项使用 LC-MS/MS 报告大量未经选择的新生儿的脐带雄激素水平的研究。我们的研究结果表明，先前的研究高估了脐带雄激素水平，并且胎儿、母亲和产科因素对脐带雄激素水平的影响不同。在解释之前发布的未考虑所有这些因素的数据时应谨慎。
The aim of this study was to measure umbilical blood androgen concentrations in a birth cohort using a highly specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay and assesses the effects of sex, labor, and gestational age on fetal androgen levels at birth. We performed a prospective cohort study of androgen concentrations in mixed arterial and venous umbilical cord serum from 803 unselected singleton pregnancies from a general obstetric population in Western Australia. Total testosterone (TT), Δ4-androstenedione, and dehydroepiandrosterone were extracted from archived cord serum samples and measured using LC-MS/MS. SHBG was measured by ELISA; free testosterone (FT) and bioavailable testosterone (BioT) values were also calculated. Median values for all three androgens were generally lower than previously published values. Levels of TT, FT, BioT, and SHBG were significantly higher in male verses female neonates (P<0.0001), while dehydroepiandrosterone levels were higher in females (P<0.0001). Labor was associated with a significant (∼15–26%) decrease in median cord blood TT and FT levels (both sexes combined), but a modest (∼16–31%) increase in SHBG, Δ4-androstenedione, and dehydroepiandrosterone concentrations. TT and FT were significantly negatively correlated with gestational age at delivery, while SHBG, Δ4-androstenedione, and dehydroepiandrosterone were positively correlated. Antenatal glucocorticoid administration also had a significant effect in the multiple regression models. This is the first study to report umbilical cord androgen levels in a large unselected population of neonates using LC-MS/MS. Our findings suggest that previous studies have over-estimated cord androgen levels, and that fetal, maternal, and obstetric factors influence cord androgen levels differentially. Caution should be exercised when interpreting previously-published data that have not taken all of these factors into account.
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影响因子：
8
作者：
Krogh, Camilla;Cohen, Arieh S.;Fischer, Thea K.
通讯作者：
Fischer, Thea K.
影响因子：
4.9
作者：
Maffeis, C;Moghetti, P;Tatò, L
通讯作者：
Tatò, L
影响因子：
5.8
作者：
Anderson, Helen;Fogel, Naomi;Dunaif, Andrea
通讯作者：
Dunaif, Andrea
影响因子：
3.8
作者：
LESLIE, KK;ZUCKERMAN, DJ;ALBERTSON, BD
通讯作者：
ALBERTSON, BD
DOI：
10.1016/j.jsbmb.2009.10.012
发表时间：
2010-08-01
影响因子：
4.1
作者：
Hill, Martin;Parizek, Antonin;Starka, Luboslav
通讯作者：
Starka, Luboslav