Vertical nanowire electrode arrays as a scalable platform for intracellular interfacing to neuronal circuits.
Vertical nanowire electrode arrays as a scalable platform for intracellular interfacing to neuronal circuits.
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DOI：
10.1038/nnano.2011.249
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发表时间：
2012-01-10
影响因子：
38.3
通讯作者：
中科院分区：
文献类型：
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破译神经元密码——神经元回路存储和处理信息的规则——是一项重大的科学挑战。目前，由于缺乏足够灵敏的实验工具来量化单个突触连接的强度，并且还具有足够的可扩展性，可以同时测量和控制大量哺乳动物神经元的单细胞分辨率，这些努力受到阻碍。在这里，我们报告了一种基于垂直纳米线的可扩展细胞内电极平台，该平台可为多个哺乳动物神经元提供并行电接口。具体来说，我们表明我们的垂直纳米线电极阵列（vnea）可以在细胞内记录和刺激大鼠皮质神经元分离培养中的神经元活动，也可以用来绘制多个单独的突触连接。该平台的可扩展性，结合其与硅纳米制造技术的兼容性，为同时实现数百个单个神经元的高保真接口提供了一条清晰的道路。
Deciphering the neuronal code - the rules by which neuronal circuits store and process information - is a major scientific challenge. Currently, these efforts are impeded by a lack of experimental tools that are sensitive enough to quantify the strength of individual synaptic connections and also scalable enough to simultaneously measure and control a large number of mammalian neurons with single-cell resolution. Here, we report a scalable intracellular electrode platform based on vertical nanowires that affords parallel electrical interfacing to multiple mammalian neurons. Specifically, we show that our vertical nanowire electrode arrays (VNEAs) can intracellularly record and stimulate neuronal activity in dissociated cultures of rat cortical neurons and can also be used to map multiple individual synaptic connections. The scalability of this platform, combined with its compatibility with silicon nanofabrication techniques, provides a clear path toward simultaneous, high-fidelity interfacing with hundreds of individual neurons.
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影响因子：
41.2
作者：
通讯作者：
--
影响因子：
64.8
作者：
Bock, Davi D.;Lee, Wei-Chung Allen;Kerlin, Aaron M.;Andermann, Mark L.;Hood, Greg;Wetzel, Arthur W.;Yurgenson, Sergey;Soucy, Edward R.;Kim, Hyon Suk;Reid, R. Clay
通讯作者：
Reid, R. Clay
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作者：
Kole, Maarten H. P.;Stuart, Greg J.
通讯作者：
Stuart, Greg J.
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Moulton, SE;Barisci, JN;Wallace, GG
通讯作者：
Wallace, GG
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64.8
作者：
ARANCIO, O;KANDEL, ER;HAWKINS, RD
通讯作者：
HAWKINS, RD