Non-Invasive Dengue Diagnostics-The Use of Saliva and Urine for Different Stages of the Illness.
Non-Invasive Dengue Diagnostics-The Use of Saliva and Urine for Different Stages of the Illness.
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非侵入性登革热诊断——利用唾液和尿液来诊断疾病的不同阶段。
DOI：
10.3390/diagnostics11081345
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发表时间：
2021-07-26
期刊：
影响因子：
--
通讯作者：
Ng LC
中科院分区：
文献类型：
--
作者：
Humaidi M;Tien WP;Yap G;Chua CR;Ng LC
登革热的诊断在很大程度上取决于临床症状，并通常通过实验室检测通过静脉采血收集的患者血清样本中的登革热病毒来确认。这对不能接受静脉穿刺术的患者来说是一个挑战。非侵入性的登革热诊断方法有可能增强当前的登革热检测算法。在这项研究中，从2012年1月至2012年9月和2013年9月至2013年12月在两个不同的设置下收集了登革热感染患者的样本。A组样本(血液、尿液和唾液)在39名患者住院时和他们的随访期间每天采集，而B组样本(唾液)则在登革热急性期采集23名患者。在A组，从发热后第2天到第4天，血清的总阳性率最高，其次是唾液和尿液(100%/82.1%/67.9%)。从发热后第5天至第10天，血清和尿液的阳性率相似(67.4%/61.2%)，其次是唾液(51.3%)。在发热后第10天以后，在血清中检测不到DENV，但尿液和唾液的阳性率分别为56.8%和28.6%。直到发烧后32天，才能在尿液中检测到DENV。B组检测唾液中DENV的总灵敏度为32.4%/36%(RNA/NS1)。我们的结果表明，基于尿液的检测方法特别适用于晚期登革热的检测，即在血清中未检测到DENV，但在尿液中仍可检测到DENV。这为医生提供了一个潜在的工具，以便在登革热传播持续的地区发现新病例，并随后促使加强媒介控制活动。
Dengue diagnosis is largely dependent on clinical symptoms and routinely confirmed with laboratory detection of dengue virus in patient serum samples collected via phlebotomy. This presents a challenge to patients not amenable to venipuncture. Non-invasive methods of dengue diagnosis have the potential to enhance the current dengue detection algorithm. In this study, samples from dengue infected patients were collected between January 2012 until September 2012 and September 2013 until December 2013 in two different setups. Panel A samples (blood, urine, and saliva) were collected daily when the 39 patients were hospitalised and during their follow-up visits while Panel B samples (saliva) were collected from 23 patients during the acute stage of dengue. Using DENV PCR on Panel A, from day 2 to day 4 post fever onset, serum showed the best overall positivity followed by saliva and urine (100%/82.1%/67.9%). From day 5 until day 10 post fever onset, serum and urine had similar positivity (67.4%/61.2%), followed by saliva (51.3%). Beyond day 10 post fever onset, DENV was undetectable in sera, but urine and saliva showed 56.8% and 28.6% positivity, respectively. DENV in urine was detectable up until 32 days post fever. Panel B results showed overall sensitivity of 32.4%/36% (RNA/NS1) for DENV detection in saliva. Our results suggest that the urine-based detection method is useful especially for late dengue detection, where DENV is undetected in sera but still detectable in urine. This provides a potential tool for the physician to pick up new cases in an area where there is ongoing dengue transmission and subsequently prompt for intensified vector control activities.
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DOI：
10.4269/ajtmh.2010.09-0552
发表时间：
2010-05-01
影响因子：
3.3
作者：
Gregory, Christopher J.;Santiago, Luis Manuel;Tomashek, Kay M.
通讯作者：
Tomashek, Kay M.
影响因子：
4.8
作者：
Ma X;Zhen W;Yang P;Sun X;Nie W;Zhang L;Xu H;Hu K
通讯作者：
Hu K
DOI：
10.3390/pathogens2020357
发表时间：
2013-05-13
期刊：
Pathogens (Basel, Switzerland)
影响因子：
--
作者：
Moi ML;Omatsu T;Hirayama T;Nakamura S;Katakai Y;Yoshida T;Saito A;Tajima S;Ito M;Takasaki T;Akari H;Kurane I
通讯作者：
Kurane I
影响因子：
0.2
作者：
Banavar, Spoorthi Ravi;Vidya, G. S.
通讯作者：
Vidya, G. S.
影响因子：
3.8
作者：
Andries, Anne-Claire;Duong, Veasna;Buchy, Philippe
通讯作者：
Buchy, Philippe