Morphology of dopaminergic amacrine cells in the mouse retina: independence from homotypic interactions.
Morphology of dopaminergic amacrine cells in the mouse retina: independence from homotypic interactions.
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DOI：
10.1002/cne.22270
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发表时间：
2010-04-15
期刊：
影响因子：
--
通讯作者：
Reese BE
中科院分区：
文献类型：
--
作者：
Keeley PW;Reese BE
为了确定树突状形态发生时相邻多巴胺能无长突（DA）细胞之间的同型相互作用的作用，检查相对于所有相邻同型细胞的定位的单个细胞的形态。对于每个标记的细胞，重建树突状区域，计算其Voronoi域，并将两者相关联。DA细胞的树突状野被观察到是大的，稀疏的和高度不规则的。树突很容易与相邻细胞的树突重叠，没有证据表明树突平铺，也没有与同型排斥或回避相一致的指状交错。此外，直接比较树突状领域领域的相同细胞的Voronoi域面积没有证据表明树突状细胞的生长受到约束或偏置的本地分布的野生型视网膜中的同型邻居。相邻的填充细胞的过程的比较证实了他们的直接接近彼此内丛状层，表明他们不从事相互回避的coursing在不同的深度。总之，这些结果表明，DA细胞的形态发生是独立的同型相互作用。然而，在促凋亡Bax基因，这产生了DA细胞数量的四倍增加的情况下，一个小的，但显着减少树突状领域的大小得到，虽然没有那么大，将预测的密度增加。目前的结果被认为是在最近的研究中的作用，细胞粘附分子表达的DA细胞。
To determine the role of homotypic interactions between neighboring dopaminergic amacrine (DA) cells upon dendritic morphogenesis, the morphology of single cells was examined relative to the positioning of all neighboring homotypic cells. For each labeled cell, the dendritic field was reconstructed, its Voronoi domain calculated, and the two were related. The dendritic fields of DA cells were observed to be large, sparse and highly irregular. Dendrites readily overlapped those of neighboring cells, showing no evidence for dendritic tiling nor inter-digitation consistent with homotypic repulsion or avoidance. Furthermore, a direct comparison of dendritic field area to the Voronoi domain area of the same cell showed no evidence for dendritic growth being constrained or biased by the local distribution of homotypic neighbors in wild-type retinas. A comparison of the processes of adjacent filled cells confirmed their immediate proximity to one another within the inner plexiform layer, indicating that they do not engage in mutual avoidance by coursing at different depths. Together, these results suggest that the morphogenesis of DA cells is independent of homotypic interactions. However, in the absence of the pro-apoptotic Bax gene, which yields a four-fold increase in DA cell number, a small but significant reduction in dendritic field size was obtained, though not so great as would be predicted by the increase in density. The present results are considered in light of recent studies on the role of cell adhesion molecules expressed by developing DA cells.
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