Optical gearbox enabled versatile multiscale high-throughput multiphoton functional imaging.
Optical gearbox enabled versatile multiscale high-throughput multiphoton functional imaging.
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DOI：
10.1038/s41467-022-34472-6
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发表时间：
2022-11-02
影响因子：
16.6
通讯作者：
Cui, Meng
中科院分区：
文献类型：
--
作者：
Lin, Jianian;Cheng, Zongyue;Yang, Guang;Cui, Meng
为了了解生物系统的功能和机制，以高时空分辨率观察活体动物内的细胞动力学至关重要。基因编码功能指标的最新进展已将响应率显着提高到接近毫秒的时间尺度。然而，广泛使用的体内成像系统通常缺乏捕捉快速生物动力学的时间解决方案。为了广泛实现高速体内深层组织成像的能力，我们开发了一种光学变速箱。作为附加模块，光学齿轮箱可以将常见的多光子成像系统转换为通用的多尺度高通量成像应用。在这项工作中，我们展示了哺乳动物大脑中帧速率从 50 到 1000Hz 的体内 2D 和 3D 功能成像。光学齿轮箱的多功能性以及与广泛使用的成像组件的兼容性对于各种深层组织成像应用来说非常有价值。作者开发了一种光学齿轮箱来加速激光扫描显微镜，实现成像帧率从数十赫兹到1kHz的灵活调整。该技术通过小鼠大脑的体内功能成像得到验证。
To understand the function and mechanism of biological systems, it is crucial to observe the cellular dynamics at high spatiotemporal resolutions within live animals. The recent advances in genetically encoded function indicators have significantly improved the response rate to a near millisecond time scale. However, the widely employed in vivo imaging systems often lack the temporal solution to capture the fast biological dynamics. To broadly enable the capability of high-speed in vivo deep-tissue imaging, we developed an optical gearbox. As an add-on module, the optical gearbox can convert the common multiphoton imaging systems for versatile multiscale high-throughput imaging applications. In this work, we demonstrate in vivo 2D and 3D function imaging in mammalian brains at frame rates ranging from 50 to 1000 Hz. The optical gearbox’s versatility and compatibility with the widely employed imaging components will be highly valuable to a variety of deep tissue imaging applications. The authors develop an optical gearbox to accelerate the laser scanning microscopes, achieving flexible adjustment of imaging frame rate from tens of Hz to 1 kHz. The technology is validated through in vivo functional imaging of mice brains.
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影响因子：
48
作者：
Donnert, Gerald;Eggeling, Christian;Hell, Stefan W.
通讯作者：
Hell, Stefan W.
DOI：
10.1126/science.aab0810
发表时间：
2015-12-11
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Gong Y;Huang C;Li JZ;Grewe BF;Zhang Y;Eismann S;Schnitzer MJ
通讯作者：
Schnitzer MJ
影响因子：
48
作者：
Kong L;Tang J;Little JP;Yu Y;Lämmermann T;Lin CP;Germain RN;Cui M
通讯作者：
Cui M
影响因子：
48
作者：
Kannan M;Vasan G;Huang C;Haziza S;Li JZ;Inan H;Schnitzer MJ;Pieribone VA
通讯作者：
Pieribone VA
影响因子：
16.6
作者：
Dana, Hod;Marom, Anat;Paluch, Shir;Dvorkin, Roman;Brosh, Inbar;Shoham, Shy
通讯作者：
Shoham, Shy