Applications of the indole-alkaloid gramine modulate the assembly of individual members of the barley rhizosphere microbiota.
Applications of the indole-alkaloid gramine modulate the assembly of individual members of the barley rhizosphere microbiota.
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作者：
Maver M;Escudero-Martinez C;Abbott J;Morris J;Hedley PE;Mimmo T;Bulgarelli D
在根-土壤界面增殖的微生物群落统称为根际微生物群，代表着植物生长、发育和健康的未开发有益资源。为了可持续农业而合理控制微生物群的一个组成部分是确定这些群落的分子决定因素。在植物中，化感物质的生物合成是定义环境中生物体间关系的中心阶段。有趣的是，这个过程是通过驯化和育种选择来塑造的。吲哚生物碱禾本科胺是这种现象的一个典型例子，它在大麦（Hordeum vulgare L.）中广泛存在于野生基因型中，但在几个现代品种中被反选择。这促使我们研究外源施用禾本科胺如何影响在参考农业土壤中生长的两种不含禾本科的优质大麦品种的根际微生物群。高通量 16S rRNA 基因扩增子测序表明，禾谷胺的应用会干扰具有相对广泛系统发育分配的土壤微生物子集的增殖。引人注目的是，这些细菌的生长似乎被大麦植物以与基因型和剂量无关的方式拯救。与此同时，我们发现宿主招募线索可以以宿主基因型依赖的方式干扰禾本科应用的影响。有趣的是，后一种效应对变形菌门成员表现出偏见。这些初步观察表明，禾谷胺可以作为根-土界面上原核生物群落的决定因素。
Microbial communities proliferating at the root-soil interface, collectively referred to as the rhizosphere microbiota, represent an untapped beneficial resource for plant growth, development and health. Integral to a rational manipulation of the microbiota for sustainable agriculture is the identification of the molecular determinants of these communities. In plants, biosynthesis of allelochemicals is centre stage in defining inter-organismal relationships in the environment. Intriguingly, this process has been moulded by domestication and breeding selection. The indole-alkaloid gramine, whose occurrence in barley (Hordeum vulgare L.) is widespread among wild genotypes but has been counter selected in several modern varieties, is a paradigmatic example of this phenomenon. This prompted us to investigate how exogenous applications of gramine impacted on the rhizosphere microbiota of two, gramine-free, elite barley varieties grown in a reference agricultural soil. High throughput 16S rRNA gene amplicon sequencing revealed that applications of gramine interfere with the proliferation of a subset of soil microbes with a relatively broad phylogenetic assignment. Strikingly, growth of these bacteria appeared to be rescued by barley plants in a genotype- and dosage-independent manner. In parallel, we discovered that host recruitment cues can interfere with the impact of gramine application in a host genotype-dependent manner. Interestingly, this latter effect displayed a bias for members of the phyla Proteobacteria. These initial observations indicate that gramine can act as a determinant of the prokaryotic communities inhabiting the root-soil interface.
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DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
3.8
作者：
Grün, S;Frey, M;Gierl, A
通讯作者：
Gierl, A
影响因子：
3.8
作者：
CORCUERA, LJ
通讯作者：
CORCUERA, LJ
影响因子：
4.8
作者：
Clark, JS
通讯作者：
Clark, JS