Temporal dynamics of the plasma microbiome in recipients at early post-liver transplantation: a retrospective study.
Temporal dynamics of the plasma microbiome in recipients at early post-liver transplantation: a retrospective study.
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DOI：
10.1186/s12866-021-02154-w
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发表时间：
2021-04-06
期刊：
影响因子：
4.2
通讯作者：
Ito Y
中科院分区：
文献类型：
--
作者：
Okumura T;Horiba K;Kamei H;Takeuchi S;Suzuki T;Torii Y;Kawada JI;Takahashi Y;Ogura Y;Ogi T;Ito Y
肝移植过程中的免疫抑制可以预防和治疗器官排斥反应，但存在严重感染性疾病的风险。免疫调节和抗菌剂影响血浆微生物群。因此，确定免疫抑制对微生物群的影响对于了解免疫活性、阐明感染源和预测移植受者的感染风险可能是重要的。我们研究了肝移植术后早期受者的血浆微生物组，并评估了这些微生物组与临床事件之间的关系。在这项研究中，纳入了2016年至2018年在名古屋大学医院接受肝移植的51名患者。分别于肝移植后1周内、肝移植后1 周内4 ± 、肝移植后1 周内8 ± 、血培养阳性后2 天内采集血浆标本。使用PATHDET管道的鸟枪式下一代测序(NGS)对111个血浆样本进行了分析。Anelloviridae、诺卡菌科、微细菌科和肠杆菌科的相对丰度在术后期间发生了显著变化。移植后1周内的微生物多样性高于移植后8 的多样性。抗菌药物与移植受者的微生物群显著相关。此外，急性细胞排斥反应(ACR)患者的肠杆菌科细菌比例明显高于非ACR患者，血浆微生物组多样性明显低于非ACR患者。从血培养中分离的细菌的测序读数在16份样本中有8份以NGS为主，在1名临床情况严重的受者中，人类疱疹病毒6被检测到是一种致病病原体。元基因组NGS技术在揭示血浆微生物组方面具有巨大的潜力，在临床环境中作为一种综合的诊断程序是有用的。特定微生物的时间动态可作为间接标记物用于判断移植受者的免疫活性和ACR。网上版载有补充材料，可在10.1186/s12866-021-02154-w查阅。
Immunosuppression during liver transplantation (LT) enables the prevention and treatment of organ rejection but poses a risk for severe infectious diseases. Immune modulation and antimicrobials affect the plasma microbiome. Thus, determining the impact of immunosuppression on the microbiome may be important to understand immunocompetence, elucidate the source of infection, and predict the risk of infection in LT recipients. We characterized the plasma microbiome of LT recipients at early post-LT and assessed the association between the microbiome and clinical events. In this study, 51 patients who received LT at Nagoya University Hospital from 2016 to 2018 were enrolled. Plasma samples were retrospectively collected at the following time points: 1) within a week after LT; 2) 4 ± 1 weeks after LT; 3) 8 ± 1 weeks after LT; and 4) within 2 days after a positive blood culture. A total of 111 plasma samples were analyzed using shotgun next-generation sequencing (NGS) with the PATHDET pipeline. Relative abundance of Anelloviridae, Nocardiaceae, Microbacteriaceae, and Enterobacteriaceae significantly changed during the postoperative period. Microbiome diversity was higher within a week after LT than that at 8 weeks after LT. Antimicrobials were significantly associated with the microbiome of LT recipients. In addition, the proportion of Enterobacteriaceae was significantly increased and the plasma microbiome diversity was significantly lower in patients with acute cellular rejection (ACR) than non-ACR patients. Sequencing reads of bacteria isolated from blood cultures were predominantly identified by NGS in 8 of 16 samples, and human herpesvirus 6 was detected as a causative pathogen in one recipient with severe clinical condition. The metagenomic NGS technique has great potential in revealing the plasma microbiome and is useful as a comprehensive diagnostic procedure in clinical settings. Temporal dynamics of specific microorganisms may be used as indirect markers for the determination of immunocompetence and ACR in LT recipients. The online version contains supplementary material available at 10.1186/s12866-021-02154-w.
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影响因子：
4.6
作者：
Mizrahi H;Peretz A;Lesnik R;Aizenberg-Gershtein Y;Rodríguez-Martínez S;Sharaby Y;Pastukh N;Brettar I;Höfle MG;Halpern M
通讯作者：
Halpern M
DOI：
10.1016/s1499-3872(11)60124-0
发表时间：
2012-02-01
影响因子：
3.3
作者：
Wu, Zhong-Wen;Ling, Zong-Xin;Li, Lan-Juan
通讯作者：
Li, Lan-Juan
短期抗生素治疗对人类喉咙和肠道微生物群有不同的长期影响。
影响因子：
3.7
作者：
Jakobsson HE;Jernberg C;Andersson AF;Sjölund-Karlsson M;Jansson JK;Engstrand L
通讯作者：
Engstrand L
影响因子：
3.7
作者：
Görzer I;Jaksch P;Kundi M;Seitz T;Klepetko W;Puchhammer-Stöckl E
通讯作者：
Puchhammer-Stöckl E
影响因子：
64.5
作者：
De Vlaminck I;Khush KK;Strehl C;Kohli B;Luikart H;Neff NF;Okamoto J;Snyder TM;Cornfield DN;Nicolls MR;Weill D;Bernstein D;Valantine HA;Quake SR
通讯作者：
Quake SR