Promising Antibiofilm Agents: Recent Breakthrough against Biofilm Producing Methicillin-Resistant Staphylococcus aureus.
Promising Antibiofilm Agents: Recent Breakthrough against Biofilm Producing Methicillin-Resistant Staphylococcus aureus.
复制标题
DOI：
10.3390/antibiotics9100667
复制
发表时间：
2020-10-03
期刊：
影响因子：
--
通讯作者：
Bendary MM
中科院分区：
文献类型：
--
作者：
Abd El-Hamid MI;Y El-Naenaeey ES;M Kandeel T;Hegazy WAH;Mosbah RA;Nassar MS;Bakhrebah MA;Abdulaal WH;Alhakamy NA;Bendary MM
耐甲氧西林金黄色葡萄球菌（MRSA）是一种引起严重疾病的超级细菌病原体。抗生素治疗对由这种耐药病原体引起的感染缺乏有效性的主要原因之一是MRSA生物膜的不稳定性，这导致世界范围内日益严重的情况。因此，迫切需要开发创新的生物膜抑制剂来控制该病原体的生物膜形成。因此，在这项工作中，我们试图评估一些有前途的生物膜抑制剂，如氧化锌纳米颗粒（ZnO NPs），蛋白酶K和金缕梅单宁（HAM）在管理MRSA生物膜的能力。使用不同的表型和基因型方法来鉴定产生生物膜的MDR MRSA分离株和所使用的有希望的试剂的生物膜/抗微生物活性。我们的研究证明了ZnO纳米颗粒，蛋白酶K和HAM对MRSA生物膜的强烈生物膜活性，沿着它们对生物膜（细胞间粘附A，icaA）和群体感应（QS）（agr）基因的转录调节。有趣的是，只有ZnO纳米颗粒对这种病原体表现出强大的抗微生物活性。总的来说，我们观察到生物膜的生产和抗菌素耐药性/agr基因型II和IV之间的总体正相关性。同时，毒素基因与生物被膜的产生之间没有明显的相关性。ZnO纳米颗粒被推荐单独用作有效的抗微生物剂和抗MDR MRSA及其生物膜相关疾病的生物膜剂。另一方面，蛋白酶-K和HAM可以与其他抗微生物剂共同施用以管理此类感染。
Multidrug resistant (MDR) methicillin-resistant Staphylococcus aureus (MRSA) is a superbug pathogen that causes serious diseases. One of the main reasons for the lack of the effectiveness of antibiotic therapy against infections caused by this resistant pathogen is the recalcitrant nature of MRSA biofilms, which results in an increasingly serious situation worldwide. Consequently, the development of innovative biofilm inhibitors is urgently needed to control the biofilm formation by this pathogen. In this work, we thus sought to evaluate the biofilm inhibiting ability of some promising antibiofilm agents such as zinc oxide nanoparticles (Zno NPs), proteinase K, and hamamelitannin (HAM) in managing the MRSA biofilms. Different phenotypic and genotypic methods were used to identify the biofilm producing MDR MRSA isolates and the antibiofilm/antimicrobial activities of the used promising agents. Our study demonstrated strong antibiofilm activities of ZnO NPs, proteinase K, and HAM against MRSA biofilms along with their transcriptional modulation of biofilm (intercellular adhesion A, icaA) and quorum sensing (QS) (agr) genes. Interestingly, only ZnO NPs showed a powerful antimicrobial activity against this pathogen. Collectively, we observed overall positive correlations between the biofilm production and the antimicrobial resistance/agr genotypes II and IV. Meanwhile, there was no significant correlation between the toxin genes and the biofilm production. The ZnO NPs were recommended to be used alone as potent antimicrobial and antibiofilm agents against MDR MRSA and their biofilm-associated diseases. On the other hand, proteinase-K and HAM can be co-administrated with other antimicrobial agents to manage such types of infections.
登录
查看更多内容
影响因子：
3.3
作者：
Bardiau, Marjorie;Detilleux, Johann;Ote, Isabelle
通讯作者：
Ote, Isabelle
影响因子：
4.6
作者：
Brackman G;Breyne K;De Rycke R;Vermote A;Van Nieuwerburgh F;Meyer E;Van Calenbergh S;Coenye T
通讯作者：
Coenye T
影响因子：
2.4
作者：
Yoon HJ;Choi JY;Lee K;Yong D;Kim JM;Song YG
通讯作者：
Song YG
影响因子：
5.2
作者：
Broussou, Diane C.;Lacroix, Marlene Z.;Ferran, Aude A.
通讯作者：
Ferran, Aude A.
影响因子：
2.2
作者：
Ciftci, Alper;Findik, Arzu;Savasan, Serap
通讯作者：
Savasan, Serap