Variable microbiomes between mosquito lines are maintained across different environments.
Variable microbiomes between mosquito lines are maintained across different environments.
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DOI：
10.1371/journal.pntd.0011306
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发表时间：
2023-09
影响因子：
3.8
通讯作者：
中科院分区：
文献类型：
--
作者：
在大多数生物体中，微生物群的组成是由环境和宿主共同决定的，但在埃及伊蚊中，宿主在微生物群形成中的作用鲜为人知。在此之前，我们已经证明了Ae的四行。埃及伊蚊在相同的条件下饲养在同一种昆虫中，其微生物群落也不同。为了确定这些品系是否在不同的时间和不同的环境中彼此不同，我们对相同的四个品系的微生物群进行了表征。埃及伊蚊是在最初的昆虫养殖场和另一个机构饲养的。虽然很明显，环境影响了这些品系的微生物群落，但我们确实观察到了每种昆虫中不同品系之间的微生物群落的明显差异。在α多样性、β多样性和特定细菌分类群的丰度方面观察到明显的差异。为了确定微生物群系中的品系特异性差异是否在不同环境中保持不变，对不同昆虫之间的分类群差异丰度进行了成对的比较。品系与来自同一昆虫的其他品系最相似，而不是与在不同昆虫中饲养的同一品系最相似。此外，在昆虫之间鉴定的不同品系之间的差异丰富的类群相对较少，这表明微生物群的品系特有属性在不同环境中不是保守的，或者每个昆虫体内有不同的微生物区系。总体而言，这些结果表明，在相同的环境条件下，蚊子品系在微生物多样性的环境中具有不同的微生物群，宿主与微生物的相互作用影响微生物群的组成和丰度取决于环境中可用细菌。蚊子微生物群在形成与人类病原体的相互作用方面发挥着关键作用。由于蚊子微生物群在病原体相互作用中的作用以及控制策略的成功制定，有助于形成蚊子微生物群组成的因素非常重要。在其他生物中，宿主和环境都会塑造微生物群的组成，但宿主在塑造蚊子微生物群中的作用还不太清楚。此前，我们已经证明，在相同的环境中饲养时，不同品系的埃及伊蚊拥有不同的微生物群。我们好奇地想知道，在同一种昆虫中再过几代，以及在不同昆虫中的不同环境中，这些差异是否仍然可以被检测到。我们发现，在每种昆虫中，这些品系的微生物群不同，这表明寄主和环境在建立微生物群中都起到了作用。结果表明，不同品系的Ae.埃及伊蚊将以不同的方式与环境相互作用，以塑造他们的微生物群，这可能会影响与人类病原体的相互作用和/或控制策略的有效性。更广泛地说，我们的结果对宿主-微生物相互作用的生态学很有意义。
The composition of the microbiome is shaped by both environment and host in most organisms, but in the mosquito Aedes aegypti the role of the host in shaping the microbiome is poorly understood. Previously, we had shown that four lines of Ae. aegypti harbored different microbiomes when reared in the same insectary under identical conditions. To determine whether these lines differed from each other across time and in different environments, we characterized the microbiome of the same four lines of Ae. aegypti reared in the original insectary and at another institution. While it was clear that the environment influenced the microbiomes of these lines, we did still observe distinct differences in the microbiome between lines within each insectary. Clear differences were observed in alpha diversity, beta diversity, and abundance of specific bacterial taxa. To determine if the line specific differences in the microbiome were maintained across environments, pair-wise differential abundances of taxa was compared between insectaries. Lines were most similar to other lines from the same insectary than to the same line reared in a different insectary. Additionally, relatively few differentially abundant taxa identified between pairs of lines were shared across insectaries, indicating that line specific properties of the microbiome are not conserved across environments, or that there were distinct microbiota within each insectary. Overall, these results demonstrate that mosquito lines under the same environmental conditions have different microbiomes across microbially- diverse environments and host by microbe interactions affecting microbiome composition and abundance is dependent on environmentally available bacteria. The mosquito microbiome plays a critical role in shaping interactions with human pathogens. The factors that contribute to shaping the composition of the mosquito microbiome are of high importance due to its role in pathogen interactions and the successful development of control strategies. In other organisms, both host and environment shape the microbiome composition, but the role of the host in shaping the mosquito microbiome is less clear. Previously, we have shown that different lines of Aedes aegypti harbor different microbiomes when reared in the same environment. We were curious to see if these differences could still be detected after further generations in the same insectary and across environments in a different insectary. We found that the microbiome differed between these lines in each insectary indicating both host and environment play a role in establishing the microbiome. Our results indicate that different lines of Ae. aegypti will interact with their environment differently to shape their microbiome, which could potentially influence interactions with human pathogens and/or the effectiveness of control strategies. More broadly, our results are of interest for the ecology of host-microbe interactions.
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影响因子：
14.9
作者：
Klindworth A;Pruesse E;Schweer T;Peplies J;Quast C;Horn M;Glöckner FO
通讯作者：
Glöckner FO
影响因子：
3.2
作者：
Ratcliffe NA;Furtado Pacheco JP;Dyson P;Castro HC;Gonzalez MS;Azambuja P;Mello CB
通讯作者：
Mello CB
DOI：
10.1038/s41396-020-00763-3
发表时间：
2021-01
期刊：
The ISME journal
影响因子：
--
作者：
Kozlova EV;Hegde S;Roundy CM;Golovko G;Saldaña MA;Hart CE;Anderson ER;Hornett EA;Khanipov K;Popov VL;Pimenova M;Zhou Y;Fovanov Y;Weaver SC;Routh AL;Heinz E;Hughes GL
通讯作者：
Hughes GL
影响因子：
15.5
作者：
Davis, Nicole M;Proctor, Diana M;Callahan, Benjamin J
通讯作者：
Callahan, Benjamin J
影响因子：
2.8
作者：
Saldaña MA;Hegde S;Hughes GL
通讯作者：
Hughes GL