Effects of long-term exposure of gelatinated and non-gelatinated cadmium telluride quantum dots on differentiated PC12 cells.
Effects of long-term exposure of gelatinated and non-gelatinated cadmium telluride quantum dots on differentiated PC12 cells.
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DOI：
10.1186/1477-3155-10-4
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发表时间：
2012-01-20
影响因子：
10.2
通讯作者：
Rochev Y
中科院分区：
文献类型：
--
作者：
Prasad BR;Mullins G;Nikolskaya N;Connolly D;Smith TJ;Gérard VA;Byrne SJ;Davies GL;Gun'ko YK;Rochev Y
未修饰的量子点（QD）的固有毒性是其在生物应用中使用的主要障碍。为了使它们更有效的神经假体和神经治疗剂，巯基乙酸（TGA）帽的碲化镉量子点，涂有明胶层，并在这项研究中与分化的嗜铬细胞瘤12（PC 12）细胞。在长达17天的孵育期后，通过MTT和APOTOX-Glo Triplex测定沿着使用共聚焦显微镜来研究QD -细胞相互作用。在分化过程中和神经突生长后，长期暴露于（长达17天）凝胶化TGA-封端的CdTe量子点的PC 12细胞导致与非凝胶化TGA-封端的CdTe量子点相比细胞毒性显著降低。量子点的毒性机制被确定为半胱天冬酶介导的细胞凋亡，由于镉从量子点的核心泄漏。因此得出结论，QD表面上的明胶封端在长期（长达17天）内充当了毒性离子从核心QD泄漏的屏障。
The inherent toxicity of unmodified Quantum Dots (QDs) is a major hindrance to their use in biological applications. To make them more potent as neuroprosthetic and neurotherapeutic agents, thioglycolic acid (TGA) capped CdTe QDs, were coated with a gelatine layer and investigated in this study with differentiated pheochromocytoma 12 (PC12) cells. The QD - cell interactions were investigated after incubation periods of up to 17 days by MTT and APOTOX-Glo Triplex assays along with using confocal microscopy. Long term exposure (up to 17 days) to gelatinated TGA-capped CdTe QDs of PC12 cells in the course of differentiation and after neurites were grown resulted in dramatically reduced cytotoxicity compared to non-gelatinated TGA-capped CdTe QDs. The toxicity mechanism of QDs was identified as caspase-mediated apoptosis as a result of cadmium leaking from the core of QDs. It was therefore concluded that the gelatine capping on the surface of QDs acts as a barrier towards the leaking of toxic ions from the core QDs in the long term (up to 17 days).
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影响因子：
15
作者：
Duan, Hongwei;Nie, Shuming
通讯作者：
Nie, Shuming
影响因子：
56.9
作者：
Bruchez, M;Moronne, M;Alivisatos, AP
通讯作者：
Alivisatos, AP
影响因子：
3.4
作者：
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通讯作者：
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影响因子：
3.3
作者：
Halliwell, B
通讯作者：
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影响因子：
3.5
作者：
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通讯作者：
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