Serum anti-SERPINE1 antibody as a potential biomarker of acute cerebral infarction.
Serum anti-SERPINE1 antibody as a potential biomarker of acute cerebral infarction.
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DOI：
10.1038/s41598-021-01176-8
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发表时间：
2021-11-05
影响因子：
4.6
通讯作者：
Iwadate Y
中科院分区：
文献类型：
--
作者：
Kubota M;Yoshida Y;Kobayashi E;Matsutani T;Li SY;Zhang BS;Mine S;Machida T;Takizawa H;Hiwasa T;Iwadate Y
动脉粥样硬化相关疾病患者血清中存在疾病特异性抗原和自身抗体已被广泛报道，并被认为是动脉壁炎症和免疫因素参与的结果。本研究的目的是确定一种新的抗体在缺血性中风患者的血清学鉴定抗原使用重组cDNA表达克隆从患者有短暂性脑缺血发作（TIA）。我们确定丝氨酸蛋白酶抑制剂，进化枝E成员1（SERPINE 1），作为候选抗原。使用扩增发光邻近均相测定-联免疫吸附测定法定量的血清抗SERPINE 1抗体水平在缺血性卒中患者中显著高于健康供体，包括急性脑梗死（aCI）、TIA和慢性脑梗死患者。抗体水平与老年、女性、高血压、糖尿病和心血管疾病密切相关。年龄、颈动脉内膜中层厚度与血清抗体水平呈正相关，提示SERPINE 1可能反映动脉粥样硬化的进展。在多变量分析中，SERPINE 1抗体水平是aCI的独立预测因子。因此，血清中抗SERPINE 1抗体水平可能作为动脉粥样硬化血栓性梗死的生物标志物。
The presence of disease-specific antigens and autoantibodies in the sera of patients with atherosclerosis-related diseases has been widely reported and is considered to result from inflammation of the arterial wall and the involvement of immune factors. The aim of this study was to identify a novel antibody in patients with ischemic stroke by serological identification of antigens using recombinant cDNA expression cloning from patients who had a transient ischemic attack (TIA). We identified the serpin peptidase inhibitor, clade E member 1 (SERPINE1), as a candidate antigen. The serum anti-SERPINE1 antibody levels quantified using amplified luminescent proximity homogeneous assay-linked immunosorbent assay were significantly higher in patients with ischemic stroke, including those with acute cerebral infarction (aCI), TIA, and chronic cerebral infarction, than in healthy donors. The antibody levels were strongly associated with old age, female sex, and presence of hypertension, diabetes mellitus, and cardiovascular disease. Age and intima-media thickness of the carotid artery were positively correlated with antibody levels, which suggests that SERPINE1 may reflect the progression of atherosclerosis. In a multivariate analysis, SERPINE1 antibody level was an independent predictor of aCI. Thus, the serum levels of anti-SERPINE1 antibody could potentially serve as a biomarker of atherothrombotic infarction.
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影响因子：
5.3
作者：
Koivistoinen, Teemu;Virtanen, Marko;Kahonen, Mika
通讯作者：
Kahonen, Mika
马来西亚受试者中纤溶酶原激活剂-1和组织纤溶酶原激活剂与2型糖尿病和代谢综合征的组织相关。
影响因子：
9.3
作者：
Al-Hamodi Z;Ismail IS;Saif-Ali R;Ahmed KA;Muniandy S
通讯作者：
Muniandy S
影响因子：
3.1
作者：
Cesari M;Pahor M;Incalzi RA
通讯作者：
Incalzi RA
DOI：
10.1073/pnas.94.5.1914
发表时间：
1997-03-04
影响因子：
11.1
作者：
Chen, YT;Scanlan, MJ;Old, LJ
通讯作者：
Old, LJ
影响因子：
8.3
作者：
Goldstein, Larry B.;Bushnell, Chair Cheryl D.;Pearson, Thomas A.
通讯作者：
Pearson, Thomas A.