Design of Dissolvable Microneedles for Delivery of a Pfs47-Based Malaria Transmission-Blocking Vaccine.
Design of Dissolvable Microneedles for Delivery of a Pfs47-Based Malaria Transmission-Blocking Vaccine.
复制标题
用于传递基于Pfs47的疟疾传播阻断疫苗的可溶微针的设计
DOI：
10.1021/acsbiomaterials.0c01363
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发表时间：
2021-05-10
影响因子：
5.8
通讯作者：
Jewell CM
中科院分区：
文献类型：
--
作者：
Yenkoidiok-Douti L;Barillas-Mury C;Jewell CM
The development of effective malaria vaccines remains a global health priority. In addition to an effective vaccine, there is urgent demand for effective delivery technologies that can be easily deployed. The need for effective vaccine delivery tools is particularly pertinent in resource-poor settings where access to healthcare is limited. Microneedles are micron-scale structures that offer distinct advantages for vaccines delivery by efficiently targeting skin-resident immune cells, eliminating injection-associated pain, and improving patient compliance. Here, we developed and characterized a candidate malaria vaccine loaded and deployed using dissolvable microneedle arrays. Of note, a newly-indicated human-relevant antigen was employed, Plasmodium falciparum surface protein P47. P47 and a potent toll-like receptor (TLR9) agonist vaccine adjuvant, CpG, were fabricated into microneedles using a gelatin polymer. Protein binding, ELISA, and fluorescence analysis confirmed the molecular structure and function of the P47 antigen and CpG were maintained after fabrication, storage, and release from microneedles. In cell culture, the cargo released from the microneedle arrays triggered TLR9 signaling and activated primary dendritic cells at levels similar to native, unincorporated vaccine components. Together, these studies demonstrate potential of microneedles as an easily-deployable strategy for a P47-based malaria vaccine.
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影响因子：
5.5
作者：
Edens C;Collins ML;Goodson JL;Rota PA;Prausnitz MR
通讯作者：
Prausnitz MR
DOI：
10.4049/jimmunol.1200601
发表时间：
2012-12-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Matthews K;Chung NP;Klasse PJ;Moore JP;Sanders RW
通讯作者：
Sanders RW
影响因子：
7.3
作者：
Kawasaki T;Kawai T
通讯作者：
Kawai T
影响因子：
81.5
作者：
Phillips, Margaret A.;Burrows, Jeremy N.;Wells, Timothy N. C.
通讯作者：
Wells, Timothy N. C.
影响因子：
10
作者：
DeMuth, Peter C.;Min, Younjin;Irvine, Darrell J.;Hammond, Paula T.
通讯作者：
Hammond, Paula T.