Effect of maternal probiotic intervention on HPA axis, immunity and gut microbiota in a rat model of irritable bowel syndrome.
Effect of maternal probiotic intervention on HPA axis, immunity and gut microbiota in a rat model of irritable bowel syndrome.
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DOI：
10.1371/journal.pone.0046051
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Hodgson DM
中科院分区：
文献类型：
--
作者：
Barouei J;Moussavi M;Hodgson DM
目的探讨母体益生菌干预对新生母体分离(MS)和(或)成年束缚应激(AS)所致Wistar大鼠脑-免疫-肠轴改变的影响。从怀孕前10天到出生后22天(断奶日)，母猪可以自由饮用添加动物双歧杆菌BB-12®(3×109cfu/m l)和简氏丙酸杆菌702(8.0×108cfu/m l)的饮用水，或控制即兴水。子代在PND 2-14期间接受多发性硬化症，或不受干扰。从PND 83到PND 85，动物每天接受30分钟的AS，或保持不受干扰作为对照。24、86天采血测定皮质酮、促肾上腺皮质激素和免疫球蛋白。分析结肠内容物中微生物区系组成和肠腔免疫球蛋白A水平。与未接触MS的动物相比，暴露于MS的动物显著增加了ACTH水平和新生儿需氧和厌氧细菌、大肠杆菌、肠球菌和梭菌的数量，但降低了血浆IgA水平。与非AS动物相比，暴露于AS的动物的ACTH和皮质酮水平显著升高，需氧细菌和双歧杆菌减少，类杆菌和大肠杆菌数量增加。与非应激成人对照组相比，MS和AS诱导的厌氧菌和梭状芽孢杆菌显著减少。母亲益生菌干预显著增加了新生儿皮质酮水平，这种水平仅在女性持续到至少12周，也导致成人ACTH水平升高和新生儿微生物区系改变，与MS相当。然而，它改善了血浆IgA反应，增加了MS成人的肠球菌和梭菌，增加了管腔IgA水平，并使成人的厌氧菌、双歧杆菌和大肠杆菌恢复正常。母亲益生菌干预导致新生儿应激途径的激活和肠道微生物区系的失衡。然而，重要的是，它改善了应激动物的免疫环境，并在一定程度上保护了成年肠道微生物群免受应激诱导的干扰。
To examine whether maternal probiotic intervention influences the alterations in the brain-immune-gut axis induced by neonatal maternal separation (MS) and/or restraint stress in adulthood (AS) in Wistar rats. Dams had free access to drinking water supplemented with Bifidobacterium animalis subsp lactis BB-12® (3×109 CFU/mL) and Propionibacterium jensenii 702 (8.0×108 CFU/mL) from 10 days before conception until postnatal day (PND) 22 (weaning day), or to control ad lib water. Offspring were subjected to MS from PND 2 to 14 or left undisturbed. From PND 83 to 85, animals underwent 30 min/day AS, or were left undisturbed as controls. On PND 24 and 86, blood samples were collected for corticosterone, ACTH and IgA measurement. Colonic contents were analysed for the composition of microflora and luminal IgA levels. Exposure to MS significantly increased ACTH levels and neonatal fecal counts of aerobic and anaerobic bacteria, E. coli, enterococci and clostridia, but reduced plasma IgA levels compared with non-MS animals. Animals exposed to AS exhibited significantly increased ACTH and corticosterone levels, decreased aerobic bacteria and bifidobacteria, and increased Bacteroides and E. coli counts compared to non-AS animals. MS coupled with AS induced significantly decreased anaerobes and clostridia compared with the non-stress adult controls. Maternal probiotic intervention significantly increased neonatal corticosterone levels which persisted until at least week 12 in females only, and also resulted in elevated adult ACTH levels and altered neonatal microflora comparable to that of MS. However, it improved plasma IgA responses, increased enterococci and clostridia in MS adults, increased luminal IgA levels, and restored anaerobes, bifidobacteria and E. coli to normal in adults. Maternal probiotic intervention induced activation of neonatal stress pathways and an imbalance in gut microflora. Importantly however, it improved the immune environment of stressed animals and protected, in part, against stress-induced disturbances in adult gut microflora.
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影响因子：
3.1
作者：
Israeli, Eran;Hershcovici, Tiberiu;Goldin, Eran
通讯作者：
Goldin, Eran
DOI：
10.1097/01.mpg.0000226376.95623.9f
发表时间：
2006-07-01
影响因子：
2.9
作者：
Garcia-Rodenas, Clara L.;Bergonzelli, Gabriela E.;Corthesy-Theulaz, Irene
通讯作者：
Corthesy-Theulaz, Irene
影响因子：
4.2
作者：
Eutamene, Helene;Lamine, Florence;Bueno, Lionel
通讯作者：
Bueno, Lionel
影响因子：
24.5
作者：
Gareau, Melanie G.;Jury, Jennifer;Perdue, Mary H.
通讯作者：
Perdue, Mary H.
影响因子：
4.2
作者：
Inoue, R;Ushida, K
通讯作者：
Ushida, K