Evidence for conserved DNA and histone H3 methylation reprogramming in mouse, bovine and rabbit zygotes.
Evidence for conserved DNA and histone H3 methylation reprogramming in mouse, bovine and rabbit zygotes.
复制标题
DOI：
10.1186/1756-8935-1-8
复制
发表时间：
2008-11-03
影响因子：
3.9
通讯作者：
Walter J
中科院分区：
文献类型：
--
作者：
Lepikhov K;Zakhartchenko V;Hao R;Yang F;Wrenzycki C;Niemann H;Wolf E;Walter J
在哺乳动物中，亲代基因组在受精后进行表观遗传重编程。这种重编程包括在受精卵中DNA复制之前，父本（精子衍生的）染色体的快速去甲基化。这种活跃的DNA去甲基化在受精卵中已经在几种哺乳动物物种中被记录，包括小鼠、大鼠、猪、人和牛，但质疑是否发生在兔中。当比较5-甲基-胞嘧啶，H3 K4 me 3和H3 K9 me 2修饰的抗体的免疫组化模式时，我们在小鼠，牛和兔受精卵中观察到相似的原核分布和动力学。在兔子中，父染色体的DNA去甲基化发生在稍晚的原核阶段。我们还发现，兔卵母细胞核移植后迅速脱甲基供体成纤维细胞的DNA。我们的数据显示，在原核成熟过程中的表观遗传重编程的主要事件，包括主动DNA去甲基化的机制，显然是保守的哺乳动物物种。
In mammals the parental genomes are epigenetically reprogrammed after fertilization. This reprogramming includes a rapid demethylation of the paternal (sperm-derived) chromosomes prior to DNA replication in zygotes. Such active DNA demethylation in the zygote has been documented for several mammalian species, including mouse, rat, pig, human and cow, but questioned to occur in rabbit. When comparing immunohistochemical patterns of antibodies against 5-methyl-cytosine, H3K4me3 and H3K9me2 modifications we observe similar pronuclear distribution and dynamics in mouse, bovine and rabbit zygotes. In rabbit DNA demethylation of the paternal chromosomes occurs at slightly advanced pronuclear stages. We also show that the rabbit oocyte rapidly demethylates DNA of donor fibroblast after nuclear transfer. Our data reveal that major events of epigenetic reprogramming during pronuclear maturation, including mechanisms of active DNA demethylation, are apparently conserved among mammalian species.
登录
查看更多内容
影响因子：
4.4
作者：
PRICE, DK;AYRES, JA;HARDISON, RC
通讯作者：
HARDISON, RC
影响因子：
3.8
作者：
Fulka, H;Mrazek, M;Fulka, J
通讯作者：
Fulka, J
影响因子：
2.5
作者：
Jeong, Young Sun;Yeo, Seungeun;Kang, Yong-Kook
通讯作者：
Kang, Yong-Kook
影响因子：
4.5
作者：
Farthing CR;Ficz G;Ng RK;Chan CF;Andrews S;Dean W;Hemberger M;Reik W
通讯作者：
Reik W
影响因子：
3.6
作者：
Bordignon, V;Clarke, HJ;Smith, LC
通讯作者：
Smith, LC