Multi-Omic Identification of Venom Proteins Collected from Artificial Hosts of a Parasitoid Wasp.
Multi-Omic Identification of Venom Proteins Collected from Artificial Hosts of a Parasitoid Wasp.
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DOI：
10.3390/toxins15060377
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发表时间：
2023-06-03
期刊：
影响因子：
4.2
通讯作者：
Ye G
中科院分区：
文献类型：
--
作者：
Yu K;Chen J;Bai X;Xiong S;Ye X;Yang Y;Yao H;Wang F;Fang Q;Song Q;Ye G
Habrobracon hebetor是一种寄生蜂，能够侵染许多鳞翅目幼虫。它利用毒液蛋白质引诱寄主幼虫，阻止寄主幼虫发育，在鳞翅目害虫生物防治中发挥重要作用。为了鉴定和表征其毒液蛋白，我们开发了一种使用人工宿主（ACV）的新型毒液收集方法，即，将氨基酸溶液包裹在石蜡膜中，使寄生蜂可以注射毒液。我们对从ACV和毒液储库（VRs）（对照）收集的推定毒液蛋白进行蛋白质全质谱分析。为了验证蛋白质组学数据的准确性，我们还收集了毒腺（VG），杜福尔腺（DG）和卵巢（OV），并进行转录组分析。在本文中，我们确定了204蛋白在ACV通过蛋白质组学分析，比较ACV推定的毒液蛋白与那些确定在VG，VR和DG通过蛋白质组和转录组的方法，并验证了一组使用定量实时聚合酶链反应。最终，201 ACV蛋白被鉴定为潜在的毒液蛋白。此外，我们筛选了152和148个推定的毒液蛋白质中确定的VG转录组和VR蛋白质组对ACV，发现只有26和25推定的毒液蛋白质，分别与ACV重叠。总而言之，我们的数据表明，蛋白质组分析的ACV结合蛋白质组转录组分析的其他器官/组织将提供最全面的识别真正的毒液蛋白在寄生蜂。
Habrobracon hebetor is a parasitoid wasp capable of infesting many lepidopteran larvae. It uses venom proteins to immobilize host larvae and prevent host larval development, thus playing an important role in the biocontrol of lepidopteran pests. To identify and characterize its venom proteins, we developed a novel venom collection method using an artificial host (ACV), i.e., encapsulated amino acid solution in paraffin membrane, allowing parasitoid wasps to inject venom. We performed protein full mass spectrometry analysis of putative venom proteins collected from ACV and venom reservoirs (VRs) (control). To verify the accuracy of proteomic data, we also collected venom glands (VGs), Dufour’s glands (DGs) and ovaries (OVs), and performed transcriptome analysis. In this paper, we identified 204 proteins in ACV via proteomic analysis; compared ACV putative venom proteins with those identified in VG, VR, and DG via proteome and transcriptome approaches; and verified a set of them using quantitative real-time polymerase chain reaction. Finally, 201 ACV proteins were identified as potential venom proteins. In addition, we screened 152 and 148 putative venom proteins identified in the VG transcriptome and the VR proteome against those in ACV, and found only 26 and 25 putative venom proteins, respectively, were overlapped with those in ACV. Altogether, our data suggest proteome analysis of ACV in combination with proteome–transcriptome analysis of other organs/tissues will provide the most comprehensive identification of true venom proteins in parasitoid wasps.
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影响因子：
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通讯作者：
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