Characterization of microRNA expression profiles and the discovery of novel microRNAs involved in cancer during human embryonic development.
Characterization of microRNA expression profiles and the discovery of novel microRNAs involved in cancer during human embryonic development.
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MicroRNA 表达谱的表征以及人类胚胎发育过程中与癌症相关的新型 MicroRNA 的发现
DOI：
10.1371/journal.pone.0069230
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Guo M
中科院分区：
文献类型：
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作者：
Lin Y;Zeng Y;Zhang F;Xue L;Huang Z;Li W;Guo M
microRNA（miRNAs）是一种长度约为22个核苷酸的非编码RNA分子，参与调控胚胎发育和肿瘤发生等多种重要的生理或病理过程。为了获得人类胚胎中miRNA的全面表达谱，我们使用miRNA微阵列表征了人类胚胎发育第4-6周的miRNA表达，并鉴定了50种人类胚胎特异性miRNA（HES-miRNA）。此外，我们选择了三个非保守或灵长类动物特异性的miRNAs，hsa-miR-638，-720和-1280，并检查了它们在各种正常和肿瘤组织中的表达水平。结果表明，大多数miRNAs的表达在早期人类胚胎发育过程中非常低。此外，一些非保守的或灵长类特异性的miRNAs在肿瘤和相应的正常组织样本中的表达存在显著差异，提示miRNAs与各种肿瘤的病理过程密切相关。这项研究首次全面概述了人类胚胎发育过程中miRNA的表达，并为人类早期胚胎发育与肿瘤发生之间的关系提供了直接证据。
MicroRNAs (miRNAs), approximately 22-nucleotide non-coding RNA molecules, regulate a variety of pivotal physiological or pathological processes, including embryonic development and tumorigenesis. To obtain comprehensive expression profiles of miRNAs in human embryos, we characterized miRNA expression in weeks 4-6 of human embryonic development using miRNA microarrays and identified 50 human-embryo-specific miRNAs (HES-miRNAs). Furthermore, we selected three non-conserved or primate-specific miRNAs, hsa-miR-638, -720, and -1280, and examined their expression levels in various normal and tumor tissues. The results show that expression of most miRNAs is extremely low during early human embryonic development. In addition, the expression of some non-conserved or primate-specific miRNAs is significantly different between tumor and the corresponding normal tissue samples, suggesting that the miRNAs are closely related to the pathological processes of various tumors. This study presents the first comprehensive overview of miRNA expression during human embryonic development and offers immediate evidence of the relationship between human early embryonic development and tumorigenesis.
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影响因子：
3.9
作者：
Bignold, Leon P.;Coghlan, Brian L. D.;Jersmann, Hubertus Pa.
通讯作者：
Jersmann, Hubertus Pa.
影响因子：
30.8
作者：
Bentwich, I;Avniel, A;Bentwich, Z
通讯作者：
Bentwich, Z
影响因子：
3.2
作者：
Garzon, Ramiro;Croce, Carlo M.
通讯作者：
Croce, Carlo M.
影响因子：
2.8
作者：
Castro, F. O.;Sharbati, S.;Einspanier, R.
通讯作者：
Einspanier, R.
影响因子：
8.8
作者：
通讯作者：
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