Mechanical Properties of Human Concentrated Growth Factor (CGF) Membrane and the CGF Graft with Bone Morphogenetic Protein-2 (BMP-2) onto Periosteum of the Skull of Nude Mice.
Mechanical Properties of Human Concentrated Growth Factor (CGF) Membrane and the CGF Graft with Bone Morphogenetic Protein-2 (BMP-2) onto Periosteum of the Skull of Nude Mice.
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DOI：
10.3390/ijms222111331
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发表时间：
2021-10-20
影响因子：
5.6
通讯作者：
Murata M
中科院分区：
文献类型：
--
作者：
Kabir MA;Hirakawa A;Zhu B;Yokozeki K;Shakya M;Huang B;Akazawa T;Todoh M;Murata M
浓缩生长因子（CGF）是100%血液衍生的，与血小板和生长因子交联的纤维蛋白胶。人CGF凝块通过压缩装置转化为膜，已广泛应用于临床。然而，CGF膜的机械性能还没有得到很好的表征。本研究的目的是测量人CGF膜的抗张强度，并观察其作为骨形态发生蛋白-2在颅骨异位部位的支架的行为。将CGF膜（5 × 5 × 2 mm ~ 3）或CGF/BMP-2（1.0 μg）膜移植于5周龄雄性裸鼠颅骨骨膜上，于移植后14 d取材。通过在4 °C下在盐水中浸泡7天，CGF膜的外观和尺寸几乎相同。0天和7天时的拉伸强度的平均值分别为0.24 MPa和0.26 MPa。拉伸强度和弹性模量在0、1、3和7天之间均无显著差异。CGF/BMP-2在第14天发现骨膜上骨诱导，而单独的CGF不诱导骨。这些结果表明，人CGF膜可以成为BMP-2的短期粘性纤维蛋白支架，并且可以作为自体膜保存用于手术后的伤口保护。
Concentrated growth factor (CGF) is 100% blood-derived, cross-linked fibrin glue with platelets and growth factors. Human CGF clot is transformed into membrane by a compression device, which has been widely used clinically. However, the mechanical properties of the CGF membranes have not been well characterized. The aims of this study were to measure the tensile strength of human CGF membrane and observe its behavior as a scaffold of BMP-2 in ectopic site over the skull. The tensile test of the full length was performed at the speed of 2mm/min. The CGF membrane (5 × 5 × 2 mm3) or the CGF/BMP-2 (1.0 μg) membrane was grafted onto the skull periosteum of nude mice (5-week-old, male), and harvested at 14 days after the graft. The appearance and size of the CGF membranes were almost same for 7 days by soaking at 4 °C in saline. The average values of the tensile strength at 0 day and 7 days were 0.24 MPa and 0.26 MPa, respectively. No significant differences of both the tensile strength and the elastic modulus were found among 0, 1, 3, and 7 days. Supra-periosteal bone induction was found at 14 days in the CGF/BMP-2, while the CGF alone did not induce bone. These results demonstrated that human CGF membrane could become a short-term, sticky fibrin scaffold for BMP-2, and might be preserved as auto-membranes for wound protection after the surgery.
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影响因子：
10.8
作者：
Martinez-Sanz, Elena;Ossipov, Dmitri A.;Varghese, Oommen P.
通讯作者：
Varghese, Oommen P.
DOI：
10.3390/ma14010150
发表时间：
2020-12-31
期刊：
Materials (Basel, Switzerland)
影响因子：
--
作者：
Onji K;Kabir MA;Zhu B;Yokozeki K;Saito T;Akazawa T;Murata M
通讯作者：
Murata M
影响因子：
--
作者：
Weisel JW;Litvinov RI
通讯作者：
Litvinov RI
影响因子：
2.7
作者：
Isobe K;Watanebe T;Kawabata H;Kitamura Y;Okudera T;Okudera H;Uematsu K;Okuda K;Nakata K;Tanaka T;Kawase T
通讯作者：
Kawase T
DOI：
10.1016/j.tripleo.2005.07.012
发表时间：
2006-03-01
期刊：
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY
影响因子：
--
作者：
Choukroun, J;Diss, A;Dohan, DM
通讯作者：
Dohan, DM