Human Fresh Fibrin Membrane with Bone Morphogenetic Protein-2 (BMP-2) Induces Bone Formation in the Subcutaneous Tissues of Nude Mice.
Human Fresh Fibrin Membrane with Bone Morphogenetic Protein-2 (BMP-2) Induces Bone Formation in the Subcutaneous Tissues of Nude Mice.
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DOI：
10.3390/ma14010150
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发表时间：
2020-12-31
期刊：
影响因子：
--
通讯作者：
Murata M
中科院分区：
文献类型：
--
作者：
Onji K;Kabir MA;Zhu B;Yokozeki K;Saito T;Akazawa T;Murata M
自体血纤维蛋白胶与血小板，称为浓缩生长因子（CGF），可以立即制备，只有决定离心没有添加凝血因子。与重组人BMP-2组合的胶原材料已经可商购用于临床应用。新鲜的CGF是具有润湿性和弹性的自动凝块，而大多数胶原蛋白膜来自牛或猪。新鲜CGF具有润湿性和弹性，而胶原膜是干燥材料，没有弹性。本研究的目的是观察人CGF膜的微观结构，并评价其作为rhBMP-2在裸鼠皮下组织中的递送支架的行为。使用24只裸鼠（5周龄，雄性）评估体内异位骨形成。将CGF膜作为对照组，CGF/rhBMP-2膜作为实验组，分别于移植后7、10、14 d取材。对采集的样本进行组织学检查，并进行组织形态计量学测量，以比较CGF的残留量以及新骨。由多种细胞成分和血小板组装的成熟纤维蛋白纤维与rhBMP-2膜在14天时诱导了几个骨岛和软骨，而没有CGF残留，而单独的CGF膜在10天时几乎被吸收，在14天时未能诱导骨形成。这些结果表明，新鲜的，人CGF膜可以有助于短期，粘性纤维蛋白基质的rhBMP-2的交付。
Autologous blood-derived fibrin glue with platelets, called the concentrated growth factor (CGF), can be prepared immediately by only the decided centrifuge without the addition of coagulation factors. Collagen materials combined with recombinant human BMP-2 have been commercially available for clinical use. The fresh CGF is auto-clot with wettability and elasticity, while most collagen membranes are derived from the cow or pig. The fresh CGF has wettability and elasticity, while collagen membranes are dry materials without elasticity. The aim of this study was to observe the microstructures of human CGF membrane and evaluate its behavior as a delivery scaffold of rhBMP-2 in the subcutaneous tissues of nude mice. Twenty-four nude mice (5-week-old, male) were used for the assessment of in vivo ectopic bone formation. Mice were received the CGF membrane as the controls and the CGF/rhBMP-2 membrane as the experimental group in the subcutaneous tissues, and harvested at 7, 10, and 14 days after the graft. Harvested samples were evaluated for the histological examination and the histomorphometric measurement was conducted to compare the residue of the CGF, as well as the new bone. Mature fibrin fibers assembled from multiple fibrillary elements and platelets with the rhBMP-2 membrane induced several bony islands and cartilage without residues of CGF at 14 days, while the CGF membrane alone was almost absorbed at 10 days and failed to induce bone formation at 14 days. These results demonstrated that the fresh, human CGF membrane could contribute to a short-term, sticky fibrin matrix for the delivery of rhBMP-2.
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影响因子：
2.7
作者：
Isobe K;Watanebe T;Kawabata H;Kitamura Y;Okudera T;Okudera H;Uematsu K;Okuda K;Nakata K;Tanaka T;Kawase T
通讯作者：
Kawase T
DOI：
10.1016/j.tripleo.2005.07.012
发表时间：
2006-03-01
期刊：
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY
影响因子：
--
作者：
Choukroun, J;Diss, A;Dohan, DM
通讯作者：
Dohan, DM
影响因子：
3.4
作者：
Friess, W;Uludag, H;Biron, R
通讯作者：
Biron, R
DOI：
10.1016/s0901-5027(98)80071-4
发表时间：
1998-10-01
影响因子：
2.4
作者：
Murata, M;Inoue, M;Nagai, N
通讯作者：
Nagai, N
影响因子：
4.3
作者：
Ehrenfest, David M. Dohan;Del Corso, Marco;Charrier, Jean-Baptiste
通讯作者：
Charrier, Jean-Baptiste