Impact of diurnal temperature variation on grape berry development, proanthocyanidin accumulation, and the expression of flavonoid pathway genes.

Impact of diurnal temperature variation on grape berry development, proanthocyanidin accumulation, and the expression of flavonoid pathway genes.
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DOI:
10.1093/jxb/err449
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发表时间:
2012-04
影响因子:
6.9
通讯作者:
Kennedy JA
Kennedy JA
中科院分区:
生物学1区
文献类型:
--
作者:
Cohen SD;Tarara JM;Gambetta GA;Matthews MA;Kennedy JA

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葡萄皮中原花青素(PA)积累的温度的影响知之甚少,尽管它在浆果成分和葡萄酒质量的重要性。野生葡萄(cv. Merlot)在白天冷却或在夜间加热+/-8 °C,从坐果到véraison在三个季节中,以确定温度对PA积累的影响。每个浆果的总PA含量仅在一年内变化,加热浆果中PA含量最高(1.46 mg浆果-1),冷却浆果中最低(0.97 mg浆果-1)。两年内,冷却浆果导致(-)-表没食子儿茶素作为延伸亚基的比例显着增加。在第三年,浆果的发展,PA积累,和参与类黄酮生物合成的几个基因的表达水平进行了评估。加热和冷却浆果改变了PA积累的初始速率,这与类黄酮途径中核心基因的表达密切相关。加热和冷却都改变了浆果生长和着色的速度,以及类黄酮途径中几个结构基因的表达。
Little is known about the impact of temperature on proanthocyanidin (PA) accumulation in grape skins, despite its significance in berry composition and wine quality. Field-grown grapes (cv. Merlot) were cooled during the day or heated at night by +/–8 °C, from fruit set to véraison in three seasons, to determine the effect of temperature on PA accumulation. Total PA content per berry varied only in one year, when PA content was highest in heated berries (1.46 mg berry−1) and lowest in cooled berries (0.97 mg berry−1). In two years, cooling berries resulted in a significant increase in the proportion of (–)-epigallocatechin as an extension subunit. In the third year, rates of berry development, PA accumulation, and the expression levels of several genes involved in flavonoid biosynthesis were assessed. Heating and cooling berries altered the initial rates of PA accumulation, which was correlated strongly with the expression of core genes in the flavonoid pathway. Both heating and cooling altered the rate of berry growth and coloration, and the expression of several structural genes within the flavonoid pathway.
遮光对(葡萄)黑皮诺果实中黄酮类化合物积累和模型系统提取的影响
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