Have I been here before? Complex interactions of age and test experience modulate the results of behavioural tests
Have I been here before? Complex interactions of age and test experience modulate the results of behavioural tests
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我以前来过这里吗？
DOI：
10.1016/j.bbr.2019.03.042
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发表时间：
2019
影响因子：
2.7
通讯作者：
Richter SH
中科院分区：
文献类型：
--
作者：
Von Kortzfleisch VT;Kästner N;Prange L;Kaiser S;Sachser N;Richter SH
最近，关于行为表型实验结果的可重复性的讨论已经出现。因此，一个巨大的重点放在确定那些因素，可能会限制行为数据的可重复性。由于综合表型表征可能涉及在特定时间段内重复检测同一动物，因此本研究的目的是系统研究两个潜在混淆变量（动物年龄和试验经验）的影响。为此目的，在一组测量焦虑样和探索性行为的常见行为测试（高架加迷宫、暗光测试、旷场测试、新笼测试）中评估两种不同年龄（9周对13周）的48只雄性C57 BL/6 J小鼠的行为。虽然每个年龄组中有一半的小鼠对测试电池是幼稚的，但另一半以前经历过相同的测试。除了年龄和测试经验对焦虑样和探索性行为的主要影响外，分析还揭示了这些因素之间的深刻相互作用。更确切地说，年龄的影响在有经验的小鼠中很明显，但在幼稚小鼠中却不明显。此外，以前的测试经验的影响在老年小鼠中比在年轻小鼠中更明显。这些发现清楚地表明，实验因素，如年龄和测试经验，不仅可以影响行为数据，而且还可以以复杂的交互方式影响。为了提供稳健和可重现的结果，在研究设计中系统地考虑这些因素是至关重要的。
Recently, a discussion about the reproducibility of results from behavioural phenotyping experiments has emerged. A huge emphasis has therefore been put on the identification of those factors that might limit the reproducibility of behavioural data. As a comprehensive phenotypic characterisation can involve testing of the same animal repeatedly over a specific time period, the aim of the present study was to systematically investigate effects of two potentially confounding variables, age of the animals and test experience. For this purpose, the behaviour of 48 male C57BL/6 J mice of two different ages (9 vs. 13 weeks) was assessed in a battery of common behavioural tests measuring anxiety-like and exploratory behaviour (Elevated Plus Maze, Dark-Light test, Open Field test, Novel Cage test). While half of the mice of each age group was naïve to the test battery, the other half had experienced the same tests before. Besides main effects of both age and test experience on anxiety-like and exploratory behaviour, the analysis also revealed profound interactions between these factors. More precisely, an effect of age was apparent in experienced but not in naïve mice. Furthermore, the effect of previous test experience was more pronounced in older than in younger mice. These findings clearly demonstrate that experimental factors, such as age and test experience, can influence behavioural data not just additively, but also in a complex, interactive way. To provide robust and reproducible results, it is thus fundamental to consider such factors systematically in the study design.
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DOI：
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发表时间：
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期刊：
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