The mechanistic effects of human digestion on magnesium oxide nanoparticles: implications for probiotics Lacticaseibacillus rhamnosus GG and Bifidobacterium bifidum VPI 1124.
The mechanistic effects of human digestion on magnesium oxide nanoparticles: implications for probiotics Lacticaseibacillus rhamnosus GG and Bifidobacterium bifidum VPI 1124.
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DOI：
10.1039/d2en00150k
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发表时间：
2022-12-01
影响因子：
7.3
通讯作者：
Mahler, Gretchen J.
中科院分区：
文献类型：
--
作者：
Garcia-Rodriguez, Alba;Stillwell, Allayah A.;Tochilovsky, Blake, V;Tanzman, Jacob, V;Limage, Rhodesherdeline;Kolba, Nikolai;Tako, Elad;Marques, Claudia N. H.;Mahler, Gretchen J.
纳米颗粒（NP）对人类肠道微生物群的影响由于肠道稳态与整体人类健康之间的联系而备受关注。由于金属氧化物纳米颗粒在食品工业中用作食品添加剂，人类对金属氧化物纳米颗粒的摄入量有所增加。具体地，氧化镁纳米颗粒（MgO-NP）已被描述为抗微生物和抗微生物膜。因此，在这项工作中，我们研究了食品添加剂MgO-NPs对益生菌和益生菌革兰氏阳性鼠李糖乳杆菌GG和两歧双歧杆菌VPI 1124的影响。物理化学表征表明，食品添加剂MgO由纳米颗粒（MgO-NPs）形成，并且在模拟消化后，MgO-NPs部分解离成Mg 2+。此外，发现含镁的纳米颗粒结构嵌入有机材料中。暴露于MgO-NP 4和24小时增加了两种L. rhamnosus和B.当在生物膜中时两歧，但当作为增殖细胞时不。高剂量的纳米MgO-NP显著促进了L. rhamnosus，而不是B。两歧这种影响可能主要是由于离子Mg 2+的存在。来自NP表征的证据表明，细菌/NP的相互作用是不利的，因为两种结构都带负电荷，这将产生排斥力。摄入的膳食MgO-NP可以形成不同的镁聚集体，其将到达小肠并与肠道微生物群相互作用。
The effects of nanoparticles (NPs) on the human gut microbiota are of high interest due to the link between the gut homeostasis and overall human health. The human intake of metal oxide NPs has increased due to its use in the food industry as food additives. Specifically, magnesium oxide nanoparticles (MgO-NPs) have been described as antimicrobial and antibiofilm. Therefore, in this work we investigated the effects of the food additive MgO-NPs, on the probiotic and commensal Gram-positive Lactobacillus rhamnosus GG and Bifidobacterium bifidum VPI 1124. The physicochemical characterization showed that food additive MgO is formed by nanoparticles (MgO-NPs) and after a simulated digestion, MgO-NPs partially dissociate into Mg2+. Moreover, nanoparticulate structures containing magnesium were found embedded in organic material. Exposures to MgO-NPs for 4 and 24 hours increased the bacterial viability of both L. rhamnosus and B. bifidum when in biofilms but not when as planktonic cells. High doses of MgO-NPs significantly stimulated the biofilm development of L. rhamnosus, but not B. bifidum. It is likely that the effects are primarily due to the presence of ionic Mg2+. Evidence from the NPs characterization indicate that interactions bacteria/NPs are unfavorable as both structures are negatively charged, which would create repulsive forces. Ingested dietary MgO-NPs could form different magnesium aggregates, that would reach the small intestine and interact with gut microbiota.
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