Sampling and electrophoretic analysis of segmented flow streams using virtual walls in a microfluidic device.
Sampling and electrophoretic analysis of segmented flow streams using virtual walls in a microfluidic device.
复制标题
DOI：
10.1021/ac801317t
复制
发表时间：
2008-11-01
影响因子：
7.4
通讯作者：
Kennedy, Robert T.
中科院分区：
文献类型：
--
作者：
Roman, Gregory T.;Wang, Meng;Shultz, Kristin N.;Jennings, Colin;Kennedy, Robert T.
描述了一种在非混相油流中分割的水塞取样和电泳分析方法。在该方法中，含水缓冲液和油流相互平行流动，在微制造的k形流体元件中形成稳定的虚拟壁。当含水样品堵塞在油流中与虚拟壁面接触时，发生聚结，样品被电动地转移到含水流体中。利用该虚拟壁，开发了两种通道电泳的注射方法。在第一种方法中，离散样品区流过电泳通道的入口，其中一部分通过电渗透流注入，称为“离散进样器”。采用这种方法，连续分段流至少可以连续注入800个桥塞，峰值面积RSD为5.1%。这种方法产生了多达1,050个理论板；虽然对注入器的分析表明，改进是可能的。在第二种方法中，对含水塞进行采样，使其形成连续流，流向微流体交叉式注入器，称为“分离注入器”。这种方法不需要分析每个单独的桥塞，而是允许对高频桥塞流进行周期性采样。使用该系统可以连续进行至少1000次注射，峰面积RSD为5.8%，理论板为53,500块。该方法被证明是有用的监测浓度变化的采样装置与10秒的时间分辨率。分段流中的水塞已应用于许多不同的化学操作，包括合成、分析、采样处理和采样。几乎所有这类研究都使用光学方法来分析堵头内容物。该方法提供了一种分析此类样品的新方法，并将使分段流系统的新应用成为可能。
A method for sampling and electrophoretic analysis of aqueous plugs segmented in a stream of immiscible oil is described. In the method, an aqueous buffer and oil stream flow parallel to each other to form a stable virtual wall in a microfabricated K-shaped fluidic element. As aqueous sample plugs in the oil stream make contact with the virtual wall coalescence occurs and sample is electrokinetically transferred to the aqueous stream. Using this virtual wall, two methods of injection for channel electrophoresis were developed. In the first, discrete sample zones flow past the inlet of an electrophoresis channel and a portion is injected by electroosmotic flow, termed the “discrete injector”. With this approach at least 800 plugs could be injected without interruption from a continuous segmented stream with 5.1% RSD in peak area. This method generated up to 1,050 theoretical plates; although analysis of the injector suggested that improvements may be possible. In a second method, aqueous plugs are sampled in a way that allows them to form a continuous stream that is directed to a microfluidic cross-style injector, termed the “desegmenting injector”. This method does not analyze each individual plug but instead allows periodic sampling of a high-frequency stream of plugs. Using this system at least 1000 injections could be performed sequentially with 5.8% RSD in peak area and 53,500 theoretical plates. This method was demonstrated to be useful for monitoring concentration changes from a sampling device with 10 s temporal resolution. Aqueous plugs in segmented flows have been applied to many different chemical manipulations including synthesis, assays, sampling processing and sampling. Nearly all such studies have used optical methods to analyze plug contents. This method offers a new way to analyze such samples and should enable new applications of segmented flow systems.
登录
查看更多内容
影响因子：
4
作者：
Dong, Liang;Jiang, Hongrui
通讯作者：
Jiang, Hongrui
影响因子：
7.4
作者：
He, MY;Sun, CH;Chiu, DT
通讯作者：
Chiu, DT
影响因子：
3.9
作者：
Günther, A;Jhunjhunwala, M;Jensen, KF
通讯作者：
Jensen, KF
影响因子：
56.9
作者：
Burns, MA;Johnson, BN;Burke, DT
通讯作者：
Burke, DT
影响因子：
7.4
作者：
Gadd, Jennifer C.;Kuyper, Christopher L.;Chiu, Daniel T.
通讯作者：
Chiu, Daniel T.