Bacteroides dorei dominates gut microbiome prior to autoimmunity in Finnish children at high risk for type 1 diabetes.
Bacteroides dorei dominates gut microbiome prior to autoimmunity in Finnish children at high risk for type 1 diabetes.
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DOI：
10.3389/fmicb.2014.00678
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发表时间：
2014
影响因子：
5.2
通讯作者：
Triplett EW
中科院分区：
文献类型：
--
作者：
Davis-Richardson AG;Ardissone AN;Dias R;Simell V;Leonard MT;Kemppainen KM;Drew JC;Schatz D;Atkinson MA;Kolaczkowski B;Ilonen J;Knip M;Toppari J;Nurminen N;Hyöty H;Veijola R;Simell T;Mykkänen J;Simell O;Triplett EW
过去半个世纪中，许多发达国家中 1 型糖尿病 (T1D) 等自身免疫性疾病的发病率急剧上升，芬兰和其他北欧国家的发病率尤其高。除了遗传倾向之外，环境因素被认为在这种增加中发挥着关键作用。与其他自身免疫性疾病一样，肠道微生物组被认为在控制高遗传风险儿童的 1 型糖尿病进展方面发挥着潜在作用，但我们对肠道微生物组在高遗传风险 1 型糖尿病儿童中如何发育知之甚少。在这项研究中，使用高通量 16S rRNA 基因测序确定了 76 名 T1D 高遗传风险儿童肠道微生物群的早期发育情况。从出生后 4-6 个月开始，直到 2.2 岁，每月收集芬兰图尔库同一家医院出生的儿童的粪便样本。在这 76 名儿童中，29 名血清转化为 T1D 相关自身免疫（病例），其中 22 名后来发展为 T1D，其余 47 名受试者保持健康（对照）。虽然在血清转化前低丰度物种中发现了一些显着的组成差异，但由两个密切相关的物种（Bacteroides dorei 和 Bacteroides vulgatus）组成的一个高丰度组，与血清转化前的对照相比，病例中的含量显着较高。在血清转化前，对 B. dorei/vulgatus 群丰度较高的样本进行宏基因组测序，以及更长的 16S rRNA 测序，确定该群体为 Bacteroides dorei。在病例中，B. dorei 的丰度在 7.6 个月时达到峰值，比第一个胰岛自身抗体出现早了 8 个多月，这表明微生物组的早期变化可能有助于预测遗传易感婴儿的 T1D 自身免疫。病例中 B. dorei 丰度增加的原因尚不清楚，但其时间似乎与固体食物的引入一致。
The incidence of the autoimmune disease, type 1 diabetes (T1D), has increased dramatically over the last half century in many developed countries and is particularly high in Finland and other Nordic countries. Along with genetic predisposition, environmental factors are thought to play a critical role in this increase. As with other autoimmune diseases, the gut microbiome is thought to play a potential role in controlling progression to T1D in children with high genetic risk, but we know little about how the gut microbiome develops in children with high genetic risk for T1D. In this study, the early development of the gut microbiomes of 76 children at high genetic risk for T1D was determined using high-throughput 16S rRNA gene sequencing. Stool samples from children born in the same hospital in Turku, Finland were collected at monthly intervals beginning at 4–6 months after birth until 2.2 years of age. Of those 76 children, 29 seroconverted to T1D-related autoimmunity (cases) including 22 who later developed T1D, the remaining 47 subjects remained healthy (controls). While several significant compositional differences in low abundant species prior to seroconversion were found, one highly abundant group composed of two closely related species, Bacteroides dorei and Bacteroides vulgatus, was significantly higher in cases compared to controls prior to seroconversion. Metagenomic sequencing of samples high in the abundance of the B. dorei/vulgatus group before seroconversion, as well as longer 16S rRNA sequencing identified this group as Bacteroides dorei. The abundance of B. dorei peaked at 7.6 months in cases, over 8 months prior to the appearance of the first islet autoantibody, suggesting that early changes in the microbiome may be useful for predicting T1D autoimmunity in genetically susceptible infants. The cause of increased B. dorei abundance in cases is not known but its timing appears to coincide with the introduction of solid food.
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影响因子：
30.8
作者：
Barrett, Jeffrey C.;Clayton, David G.;Concannon, Patrick;Akolkar, Beena;Cooper, Jason D.;Erlich, Henry A.;Julier, Cecile;Morahan, Grant;Nerup, Jorn;Nierras, Concepcion;Plagnol, Vincent;Pociot, Flemming;Schuilenburg, Helen;Smyth, Deborah J.;Stevens, Helen;Todd, John A.;Walker, Neil M.;Rich, Stephen S.
通讯作者：
Rich, Stephen S.
DOI：
10.1073/pnas.1002601107
发表时间：
2010-06-29
影响因子：
11.1
作者：
Dominguez-Bello, Maria G.;Costello, Elizabeth K.;Knight, Rob
通讯作者：
Knight, Rob
影响因子：
3.1
作者：
BREELING, JL;ONDERDONK, AB;KASPER, DL
通讯作者：
KASPER, DL
短期抗生素治疗对人类喉咙和肠道微生物群有不同的长期影响。
影响因子：
3.7
作者：
Jakobsson HE;Jernberg C;Andersson AF;Sjölund-Karlsson M;Jansson JK;Engstrand L
通讯作者：
Engstrand L
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y