Developmental programming of long non-coding RNAs during postnatal liver maturation in mice.
Developmental programming of long non-coding RNAs during postnatal liver maturation in mice.
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长期非编码RNA在产后肝脏成熟过程中的发育编程。
DOI：
10.1371/journal.pone.0114917
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Zhong XB
中科院分区：
文献类型：
--
作者：
Peng L;Paulson A;Li H;Piekos S;He X;Li L;Zhong XB
肝脏是重要的器官，在新陈代谢、蛋白质合成和免疫防御中起着至关重要的作用。大多数肝脏功能在出生时并不成熟，在出生后肝脏发育过程中会发生许多变化。然而，目前尚不清楚出生后肝脏会发生什么变化，发生在哪些发育阶段，以及发育过程是如何调节的。长非编码RNA(LncRNAs)参与器官发育和细胞分化。在这里，我们用核糖核酸测序的方法分析了从围生期(−2天)到成年(60天)小鼠肝脏中lncRNAs的转录组，试图了解lncRNAs在肝脏成熟中的作用。我们发现了大约15,000个基因表达，其中包括大约2,000个lncRNA。大多数lncRNAs的表达水平低于编码RNA。编码RNAs和LncRNAs都表现出三种主要的个体发生模式：在新生儿、青少年或成年阶段丰富。邻近编码和非编码RNA显示出高度相关的个体表达模式的趋势。基因本体论(GO)分析表明，一些在新生儿年龄丰富的lncRNAs具有与细胞增殖、免疫激活相关过程、组织途径和造血相关的邻近蛋白编码基因；另一些在青春期丰富的lncRNAs具有与不同代谢过程相关的邻近蛋白编码基因。这些数据揭示了出生后肝脏发育过程中显著的功能转变，并暗示了lncRNAs在肝脏成熟过程中的潜在重要性。
The liver is a vital organ with critical functions in metabolism, protein synthesis, and immune defense. Most of the liver functions are not mature at birth and many changes happen during postnatal liver development. However, it is unclear what changes occur in liver after birth, at what developmental stages they occur, and how the developmental processes are regulated. Long non-coding RNAs (lncRNAs) are involved in organ development and cell differentiation. Here, we analyzed the transcriptome of lncRNAs in mouse liver from perinatal (day −2) to adult (day 60) by RNA-Sequencing, with an attempt to understand the role of lncRNAs in liver maturation. We found around 15,000 genes expressed, including about 2,000 lncRNAs. Most lncRNAs were expressed at a lower level than coding RNAs. Both coding RNAs and lncRNAs displayed three major ontogenic patterns: enriched at neonatal, adolescent, or adult stages. Neighboring coding and non-coding RNAs showed the trend to exhibit highly correlated ontogenic expression patterns. Gene ontology (GO) analysis revealed that some lncRNAs enriched at neonatal ages have their neighbor protein coding genes also enriched at neonatal ages and associated with cell proliferation, immune activation related processes, tissue organization pathways, and hematopoiesis; other lncRNAs enriched at adolescent ages have their neighbor protein coding genes associated with different metabolic processes. These data reveal significant functional transition during postnatal liver development and imply the potential importance of lncRNAs in liver maturation.
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影响因子：
--
作者：
Li G;Zhang H;Wan X;Yang X;Zhu C;Wang A;He L;Miao R;Chen S;Zhao H
通讯作者：
Zhao H
影响因子：
10.5
作者：
Cabili, Moran N.;Trapnell, Cole;Rinn, John L.
通讯作者：
Rinn, John L.
影响因子：
10.5
作者：
Hu, Wenqian;Yuan, Bingbing;Lodish, Harvey F.
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Lodish, Harvey F.
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作者：
Kapranov, Philipp;Cheng, Jill;Gingeras, Thomas R.
通讯作者：
Gingeras, Thomas R.
人类和小鼠基因组中的复杂基因座。
影响因子：
4.5
作者：
Engstrom, Par G.;Suzuki, Harukazu;Ninomiya, Noriko;Akalin, Altuna;Sessa, Luca;Lavorgna, Giovanni;Brozzi, Alessandro;Luzi, Lucilla;Tan, Sin Lam;Yang, Liang;Kunarso, Galih;ng, Edwin Lian-Cho Ng;Batalov, Serge;Wahlestedt, Claes;Kai, Chikatoshi;Kawai, Jun;Carninci, Piero;Hayashizaki, Yoshihide;Wells, Christine;Bajic, Vladimir B.;Orlando, Valerio;Reid, James F.;Lenhard, Boris;Lipovich, Leonard
通讯作者：
Lipovich, Leonard