Differential translocation of nuclear factor-kappaB in a cardiac muscle cell line under gravitational changes.
Differential translocation of nuclear factor-kappaB in a cardiac muscle cell line under gravitational changes.
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DOI：
10.1115/1.3128718
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发表时间：
2009-06
期刊：
影响因子：
--
通讯作者：
Banerjee RK
中科院分区：
文献类型：
--
作者：
Kwon O;Tranter M;Jones WK;Sankovic JM;Banerjee RK
微重力（micro-g）环境已被证明在广泛的细胞类型测定中引起特定基因的失调。已知转录因子和分子信号通路的激活影响与应激和适应性反应相关的各种生理结果。核因子-κ B（NF-κB）是最常见的氧化敏感性转录因子之一。假设模拟微重力将激活NF-κB及其下游转录网络，从而表明NF-κB在微重力诱导的肌肉萎缩中的作用。为研究微重力条件下大鼠心肌细胞系（H9 c2）NF-κB的活化，采用旋转壁式生物反应器模拟微重力条件。Western blotting结果显示，与TNF-α阳性对照组相比，micro-g和unit-g动态对照组NF-κB p65亚基的平均核转位率分别为69%和46%（p< 0.05，n = 3）。用酶联免疫吸附试验（ELISA）证实了蛋白质印迹法的结果，与阳性对照相比，微量组为66%，动态对照组为45%（p < 0.05，n= 3）。这些结果表明在模拟微重力下NF-κB p65的显著差异易位。这些结果可以扩展到解释生理变化，如肌肉萎缩，并进一步确定在暴露于微重力下激活的调节途径和效应分子。
Microgravity (micro-g) environments have been shown to elicit dysregulation of specific genes in a wide assay of cell types. It is known that the activation of transcription factors and molecular signaling pathways influence various physiological outcomes associated with stress and adaptive responses. Nuclear factor-kappa B (NF-κB) is one of the most prevailing oxidation-sensitive transcription factors. It is hypothesized that simulated microgravity would activate NF-κB and its downstream transcriptional networks, thus suggesting a role for NF-κB in microgravity induced muscle atrophy. To investigate the activation of NF-κB in a rat cardiac cell line (H9c2) under micro-g, rotating wall vessel bioreactors were used to simulate micro-g conditions. Western blotting revealed that mean nuclear translocation of NF-κB p65 subunit was 69% for micro-g and 46% for unit-g dynamic control as compared with a 30 min TNF-α positive control (p< 0.05, n = 3). The results from western blots were confirmed by enzymelinked immunosorbent assay, which showed 66% for micro-g and 45% for dynamic control as compared with positive control (p < 0.05, n= 3). These results show significant differential translocation of NF-κB p65 under simulated micro-g. These results may be expanded upon to explain physiological changes such as muscle atrophy and further identify the regulatory pathways and effector molecules activated under exposure to micro-g.
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影响因子：
6.7
作者：
Guo, M;Sahni, SK;Francis, CW
通讯作者：
Francis, CW
影响因子：
3.9
作者：
HUGHESFULFORD, M
通讯作者：
HUGHESFULFORD, M
影响因子：
3.8
作者：
Kwon, Ohwon;Devarakonda, Surendra B.;Banerjee, Rupak K.
通讯作者：
Banerjee, Rupak K.
DOI：
10.1007/bf01404744
发表时间：
1992-01-01
期刊：
Journal of tissue culture methods : Tissue Culture Association manual of cell, tissue, and organ culture procedures
影响因子：
--
作者：
Schwarz, R P;Goodwin, T J;Wolf, D A
通讯作者：
Wolf, D A
影响因子：
5.6
作者：
Pagano, M;Naviglio, S;Illiano, G
通讯作者：
Illiano, G