An Interleukin-17 Isoform from Thick Shell Mussel Mytilus coruscus Serves as a Mediator of Inflammatory Response.
An Interleukin-17 Isoform from Thick Shell Mussel Mytilus coruscus Serves as a Mediator of Inflammatory Response.
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DOI：
10.3390/molecules28041806
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发表时间：
2023-02-14
期刊：
影响因子：
4.6
通讯作者：
Qi, Pengzhi
中科院分区：
文献类型：
--
作者：
Zhao, Jiemei;Dong, Zhenyu;Zhu, Li;Song, Weihua;Qi, Pengzhi
炎性细胞因子白介素17(IL17)通过与其受体(IL17Rs)结合，激活免疫防御信号，在先天免疫中发挥重要作用。到目前为止，有关IL17家族成员的信息在软体动物物种中仍然非常有限。在此，从厚壳贻贝中鉴定出一个新的IL17家族成员，并通过结构域预测和系统发育分析将该基因命名为McIL17-1。McIL17-1转录本在所有受检组织中均有表达，在鳃、血细胞和消化腺中高表达。不同病原体相关分子模式(PAMPs)刺激72h后，McIL17-1的转录表达显著上调，但PolyI：C刺激除外。荧光显微镜下观察到McIL17-1在HEK293T细胞中的胞质定位。此外，还进行了体内和体外实验，以评估McIL17-1在免疫应答中的潜在功能。通过RNA干扰(RNAi)和重组蛋白注射分别在体内敲除或过表达McIL17-1。在活体溶藻弧菌感染下，McIL17-1高表达组的死亡率高于对照组，而McIL17-1基因敲除组的死亡率低于对照组。体外流式细胞仪分析显示，内毒素刺激后McIL17-1抑制的血细胞凋亡率明显低于对照组。这些结果表明，新近发现的IL17亚型参与了对细菌感染的炎症反应。
The inflammatory cytokine interleukin-17 (IL17) plays an important role in innate immunity by binding to its receptors (IL17Rs) to activate immune defense signals. To date, information on members of the IL17 family is still very limited in molluscan species. Here, a novel member of the IL17 family was identified and characterized from thick shell mussel Mytilus coruscus, and this gene was designated as McIL17-1 by predicting structural domains and phylogenetic analysis. McIL17-1 transcripts existed in all examined tissues with high expression levels in gills, hemocytes and digestive glands. After the stimuli of different pathogen associated molecular patterns (PAMPs) for 72 h, transcriptional expression of McIL17-1 was significantly upregulated, except for poly I:C stimulation. Cytoplasm localization of McIL17-1 was shown in HEK293T cells by fluorescence microscopy. Further, in vivo and in vitro assays were performed to evaluate the potential function of McIL17-1 played in immune response. McIL17-1 was either knocked down or overexpressed in vivo through RNA inference (RNAi) and recombinant protein injection, respectively. With the infection of living Vibrio alginolyticus, a high mortality rate was exhibited in the McIL17-1 overexpressed group compared to the control group, while a lower mortality rate was observed in the McIL17-1 knocked down group than control group. In vitro, the flow cytometric analysis showed that the apoptosis rate of McIL17-1 inhibited hemocytes was significantly lower than that of the control group after lipopolysaccharide stimulation. These results collectively suggested that the newly identified IL17 isoform is involved in the inflammatory response to bacterial infection in M. coruscus.
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