Pathological features of COVID-19-associated lung injury: a preliminary proteomics report based on clinical samples.
Pathological features of COVID-19-associated lung injury: a preliminary proteomics report based on clinical samples.
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DOI：
10.1038/s41392-020-00355-9
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发表时间：
2020-10-15
影响因子：
39.3
通讯作者：
Zhong W
中科院分区：
文献类型：
--
作者：
Leng L;Cao R;Ma J;Mou D;Zhu Y;Li W;Lv L;Gao D;Zhang S;Gong F;Zhao L;Qiu B;Xiang H;Hu Z;Feng Y;Dai Y;Zhao J;Wu Z;Li H;Zhong W
COVID-19大流行因其与严重肺炎和相对较高的死亡率相关而成为全球卫生紧急事件。然而，COVID-19肺炎的分子特征和病理特征在很大程度上仍然未知。为了使用蛋白质组学方法表征肺组织中COVID-19发病机制的分子机制，从新死亡的COVID-19肺炎患者获得新鲜肺组织。病毒灭活后，定量蛋白质组学方法结合生物信息学分析，检测蛋白质组学变化的SARS-CoV-2感染的肺组织。我们鉴定了参与多种基本生物学过程的显著差异表达蛋白，包括细胞代谢、血液凝固、免疫应答、血管生成和细胞微环境调节。多种炎症因子表达上调，可能与NF-κB信号通路激活有关。发现了肺蛋白质组对SARS-CoV-2感染的广泛失调。我们的研究结果系统地概述了SARS-CoV-2感染肺部反应的分子病理学特征，并为控制COVID-19大流行急需的治疗靶点提供了科学依据。
The COVID-19 pandemic has emerged as a global health emergency due to its association with severe pneumonia and relative high mortality. However, the molecular characteristics and pathological features underlying COVID-19 pneumonia remain largely unknown. To characterize molecular mechanisms underlying COVID-19 pathogenesis in the lung tissue using a proteomic approach, fresh lung tissues were obtained from newly deceased patients with COVID-19 pneumonia. After virus inactivation, a quantitative proteomic approach combined with bioinformatics analysis was used to detect proteomic changes in the SARS-CoV-2-infected lung tissues. We identified significant differentially expressed proteins involved in a variety of fundamental biological processes including cellular metabolism, blood coagulation, immune response, angiogenesis, and cell microenvironment regulation. Several inflammatory factors were upregulated, which was possibly caused by the activation of NF-κB signaling. Extensive dysregulation of the lung proteome in response to SARS-CoV-2 infection was discovered. Our results systematically outlined the molecular pathological features in terms of the lung response to SARS-CoV-2 infection, and provided the scientific basis for the therapeutic target that is urgently needed to control the COVID-19 pandemic.
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影响因子：
--
作者：
Muncie JM;Weaver VM
通讯作者：
Weaver VM
影响因子：
14.9
作者：
Ma J;Chen T;Wu S;Yang C;Bai M;Shu K;Li K;Zhang G;Jin Z;He F;Hermjakob H;Zhu Y
通讯作者：
Zhu Y
影响因子：
30.5
作者：
Monticelli LA;Buck MD;Flamar AL;Saenz SA;Tait Wojno ED;Yudanin NA;Osborne LC;Hepworth MR;Tran SV;Rodewald HR;Shah H;Cross JR;Diamond JM;Cantu E;Christie JD;Pearce EL;Artis D
通讯作者：
Artis D
影响因子：
30.5
作者：
Corry, DB;Rishi, K;Kheradmand, F
通讯作者：
Kheradmand, F
影响因子：
5.3
作者：
Bober, Marta;Enochsson, Charlotte;Morgelin, Matthias
通讯作者：
Morgelin, Matthias