Connective tissue activation. XXXI. Identification of two molecular forms of a human mesenchymal cell-derived growth factor, connective tissue activating peptide-V.
Connective tissue activation. XXXI. Identification of two molecular forms of a human mesenchymal cell-derived growth factor, connective tissue activating peptide-V.
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结缔组织激活。
DOI：
10.1002/art.1780301209
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发表时间：
1987
影响因子：
--
通讯作者：
Castor,CW
中科院分区：
文献类型：
--
作者：
Cabral,AR;Castor,CW
我们以前确定，在正常尿液中，生长因子，刺激单层培养的人滑膜，软骨和真皮成纤维细胞合成增量的透明质酸，蛋白多糖和DNA。通过生物测定和免疫学方法的指导下分离程序，在还原和非还原样品中通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳的单一条带判断，发现了两种阴离子活性多肽，其Mr分别为28，000和16，000。兔抗体对每个纯化的蛋白质进行反应，免疫扩散和Western印迹，与两种抗原。免疫组织化学和免疫结合研究检测到的蛋白质在正常人滑膜，真皮，软骨成纤维细胞和人隐静脉内皮细胞。间充质细胞衍生的生长因子现在被命名为结缔组织激活肽-V（CTAP-V）。在放射免疫扩散试验中使用单特异性多克隆抗CTAP-V抗体，以定量测定生物液体中的抗原。在正常人血浆中，CTAP-V的浓度低于检测限。正常尿液中的CTAP-V浓度为4.5 ± 2.0 mg/ml，根据5-18倍浓缩样本的测量值计算。来自风湿性疾病和肾功能正常患者的关节液的CTAP-V水平与血浆中发现的水平相似;在慢性肾衰竭患者的血浆和关节液中检测到2-15倍的增加。免疫扩散或斑点印迹分析显示10种哺乳动物的血浆或血清中存在CTAP-V样物质。在2种鸟类中未检出。
We previously identified, in normal urine, a growth factor that stimulated monolayer cultures of human synovial, cartilage, and dermal fibroblasts to synthesize incremental amounts of hyaluronic acid, proteoglycans, and DNA. An isolation procedure guided by bioassays and immunologic methods disclosed 2 anionic bioactive polypeptides withMrof 28,000 and 16,000, respectively, as judged by single bands with sodium dodecyl sulfate—polyacrylamide gel electrophoresis in reduced and nonreduced samples. Rabbit antibodies raised against each purified protein were shown to react, on immunodiffusion and Western blot, with both antigens. Immunohistochemical and immunobinding studies detected the protein in normal human synovial, dermal, and cartilage fibroblasts and in human saphenous vein endothelial cells. The mesenchymal cell—derived growth factor is now designated connective tissue activating peptide—V (CTAP‐V). Monospecific polyclonal anti—CTAP‐V antibodies were used in a radial immunodiffusion assay for quantitative determination of the antigen in biologic fluids. In normal human plasma the concentration of CTAP‐V was below the limit of detection. The CTAP‐V concentration in normal urine was 4.5 ± 2.0 m̈g/ml, calculated from measurements of 5—18‐fold concentrated samples. Joint fluid from patients with rheumatic diseases and normal renal function had CTAP‐V levels similar to those found in plasma; 2—15‐fold increases were detected in plasma and joint fluid of patients with chronic renal failure. Immunodiffusion or dot‐blot analysis revealed a CTAP‐V—like material in the plasma or serum of 10 mammalian species. It was not detectable in 2 avian species.
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体外硬皮病皮肤成纤维细胞胶原合成增加：硬皮病成纤维细胞的调节或激活可能存在缺陷。
影响因子：
15.9
作者：
E. Leroy
通讯作者：
E. Leroy
肾小球滤过和蛋白尿的生物生理学。
DOI：
--
发表时间：
1984
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Kanwar,YS
通讯作者：
Kanwar,YS
从葡萄球菌蛋白 A 中回收抗原——抗体吸附剂。
影响因子：
--
作者：
J. Macsween;S. Eastwood
通讯作者：
S. Eastwood
影响因子：
--
作者：
C. Castor;Susan F. Smith;James C. Ritchie;E. Dorstewitz
通讯作者：
E. Dorstewitz
影响因子：
--
作者：
Cabral,A;Castor,CW
通讯作者：
Castor,CW