Identification of Ligands for Chicken Transient Receptor Potential Ankyrin 1 Channel and Chemosensory Perception of Herbal Compounds in Chickens.

Identification of Ligands for Chicken Transient Receptor Potential Ankyrin 1 Channel and Chemosensory Perception of Herbal Compounds in Chickens.
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DOI:
10.2141/jpsa.0210132
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发表时间:
2022-07-25
期刊:
The journal of poultry science
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其他
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由香料和草药引起的辛辣感在食物选择和食欲方面起着重要作用,并且建议在饲料中添加香料和草药作为抗生素的天然替代品对家禽养殖具有有益的影响。然而,我们的知识的化学感觉的感觉草药化合物在鸡是有限的。瞬时受体电位锚蛋白1(TRPA1)参与各种草药化合物的感觉感知。在这里,我们进行了钙成像和电生理分析,使用细胞瞬时表达鸡TRPA1(cTRPA1),并确定了两个新的cTRPA1配体丁香酚和百里酚。在行为测定中,鸡对cTRPA1配体,包括丁香酚、百里酚、肉桂醛、香芹酚和异硫氰酸烯丙酯有反应。这些结果提供的证据表明,鸡有一个功能性的TRPA1通道和各种草药化合物的化学感觉知觉。
The pungency induced by spices and herbs plays an important role in food choice and appetite, and it is suggested that adding spices and herbs to feed as natural alternatives to antibiotics has beneficial effects in poultry farming. However, our knowledge of the chemosensory perception of herbal compounds in chickens is limited. Transient receptor potential ankyrin 1 (TRPA1) is involved in the sensory perception of various herbal compounds. Here, we performed calcium imaging and electrophysiological analyses using cells transiently expressing chicken TRPA1 (cTRPA1) and identified two novel cTRPA1 ligands—eugenol and thymol. In a behavioral assay, chickens responded to cTRPA1 ligands, including eugenol, thymol, cinnamaldehyde, carvacrol, and allyl isothiocyanate. These results provide evidence that chickens have a functional TRPA1 channel and chemosensory perception of various herbal compounds.
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