Activation of reciprocal pathways between arcuate nucleus and ventrolateral periaqueductal gray during electroacupuncture: involvement of VGLUT3.
Activation of reciprocal pathways between arcuate nucleus and ventrolateral periaqueductal gray during electroacupuncture: involvement of VGLUT3.
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DOI：
10.1016/j.brainres.2010.08.102
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发表时间：
2010-11-11
期刊：
影响因子：
2.9
通讯作者：
Longhurst JC
中科院分区：
文献类型：
--
作者：
Guo ZL;Longhurst JC
电针正中神经上的健十内关穴(P5-P6)通过激活弓状核(ARC)和腹外侧导水管周围灰质(VlPAG)来减弱交感兴奋反应。在电针过程中，ARC或vlPAG的激活分别导致两个核团的神经元兴奋。然而，这两个核团之间的直接投射可能参与电针过程中的中枢神经处理，目前还没有确定。囊泡谷氨酸转运体3(VGLUT3)标记谷氨酸能神经元。因此，本研究评估了电针期间ARC和vlPAG之间的直接神经元投射，重点放在含有VGLUT3的神经元上。将罗丹明标记的微球逆行示踪剂(100nL)注入vlPAG或ARC后7~10d，电针或作为假手术对照。采用低频(2 Hz)电针双侧P5~P6穴位30min。两组ARC和vlPAG均可见含有微球示踪剂的Perikarya。与对照组(无电刺激)相比，电针治疗组大鼠ARC和vlPAG的c-Fos免疫反应和c-Fos双标神经元显著增加(P均<0.01)。此外，在电针刺激后，c-Fos、逆行示踪剂和VGLUT_3在两个核团内对一些神经元进行了三重标记(P<；0.01，两个核团)。这些结果表明，ARC和vlPAG之间的直接相互投射参与了电针对交感神经活动的延长调制，VGLUT3神经元是参与这一过程的重要神经元表型。
Electroacupuncture (EA) at the Jianshi-Neiguan acupoints (P5-P6, overlying the median nerve) attenuates sympathoexcitatory responses through activation of the arcuate nucleus (ARC) and ventrolateral periaqueductal gray (vlPAG). Activation of the ARC or vlPAG respectively leads to neuronal excitation of the both nuclei during EA. However, direct projections between these two nuclei that could participate in central neural processing during EA have not been identified. The vesicular glutamate transporter 3 (VGLUT3) marks glutamatergic neurons. Thus, the present study evaluated direct neuronal projections between the ARC and vlPAG during EA, focusing on neurons containing VGLUT3. Seven to ten days after unilateral microinjection of a rodamine-conjugated microsphere retrograde tracer (100 nl) into the vlPAG or ARC, rats were subjected to EA or served as a sham-operated control. Low frequency (2 Hz) EA was performed bilaterally for 30 min at the P5-P6 acupoints. Perikarya containing the microsphere tracer were found in the ARC and vlPAG of both groups. Compared to controls (needle placement without electrical stimulation), c-Fos immunoreactivity and neurons double-labeled with c-Fos, an immediate early gene and the tracer were increased significantly in the ARC and vlPAG of EA-treated rats (both P<0.01). Moreover, some neurons were triple-labeled with c-Fos, the retrograde tracer and VGLUT3 in the two nuclei following EA stimulation (P<0.01, both nuclei). These results suggest that direct reciprocal projections between the ARC and vlPAG are available to participate in prolonged modulation by EA of sympathetic activity and that VGLUT3-containing neurons are an important neuronal phenotype involved in this process.
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影响因子：
3.3
作者：
Fu, Liang-Wu;Longhurst, John C.
通讯作者：
Longhurst, John C.
影响因子：
3.7
作者：
Boulland, Jean-Luc;Jenstad, Monica;Chaudhry, Farrukh A.
通讯作者：
Chaudhry, Farrukh A.
影响因子：
2.9
作者：
Guo, ZL;Moazzami, AR;Longhurst, JC
通讯作者：
Longhurst, JC
影响因子：
1.7
作者：
Guo, ZL;Longhurst, JC
通讯作者：
Longhurst, JC
影响因子：
3
作者：
CHRONWALL, BM
通讯作者：
CHRONWALL, BM